Ishibashi T, Kimura H, Uchida T, Kariyone S, Friese P, Burstein S A
First Department of Internal Medicine, Fukushima Medical College, Japan.
Proc Natl Acad Sci U S A. 1989 Aug;86(15):5953-7. doi: 10.1073/pnas.86.15.5953.
The response of cells of the murine megakaryocytic lineage to human interleukin 6 (IL-6) was assessed in serum-depleted cultures using a variety of biological assays. IL-6 alone had no influence on megakaryocytic colony formation but augmented the numbers of these colonies induced by the multipotent colony-stimulating factor interleukin 3. However, in liquid marrow cultures, IL-6 alone promoted marked increments in megakaryocytic size and the activity of acetyl-cholinesterase, a marker enzyme of the lineage. Moreover, IL-6 induced a significant shift toward higher ploidy classes when megakaryocytic DNA was quantitated by flow cytometry. To determine whether the influence of IL-6 on megakaryocytic maturation was direct, the factor was added to cultures of single megakaryocytes isolated from megakaryocytic colonies. Fifty-four percent of these cells increased in size compared with 19% of those grown without IL-6. The data show that human IL-6 is a potent direct-acting growth factor for murine megakaryocytes with activity promoting maturation of that lineage.
使用多种生物学检测方法，在血清缺乏的培养体系中评估了小鼠巨核细胞系细胞对人白细胞介素6（IL-6）的反应。单独的IL-6对巨核细胞集落形成没有影响，但能增加由多能集落刺激因子白细胞介素3诱导的这些集落的数量。然而，在液体骨髓培养中，单独的IL-6能显著增加巨核细胞的大小以及乙酰胆碱酯酶的活性，乙酰胆碱酯酶是该细胞系的一种标志酶。此外，当通过流式细胞术对巨核细胞DNA进行定量时，IL-6能诱导明显向更高倍体类别的转变。为了确定IL-6对巨核细胞成熟的影响是否直接，将该因子添加到从巨核细胞集落中分离出的单个巨核细胞的培养物中。与未添加IL-6培养的细胞相比，这些细胞中有54%的细胞大小增加，而未添加IL-6培养的细胞中这一比例为19%。数据表明，人IL-6是一种对小鼠巨核细胞具有强大直接作用的生长因子，具有促进该细胞系成熟的活性。
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