Williams N, Jackson H, Ralph P, Nakoinz I
Blood. 1981 Jan;57(1):157-63.
Auxiliary bone marrow cells are required for optimal murine megakaryocyte colony formation in addition to progenitor cells and a colony stimulating activity (CSA) present in WEHI-3 cell conditioned medium. These auxiliary cells are adherent, with a sedimentation rate of 5.8 mm hr-1 and buoyant density of 1.065-1.078 gcm-3. The activity from bone marrow cells is loss at irradiation doses above 900 rad. Bone marrow cells with these characteristics, and supernatants from lung, bone shafts, and peritoneal exudate cells were all active in enhancing megakaryocyte colony incidences in mouse bone marrow cultures above those stimulated by an obligatory activity in WEHI-3 cell conditioned medium. Certain macrophage cell lines (J774, P388D1) could elaborate the activity. This study confirms that a potentiation activity enhances CSA stimulation of megakaryocyte colony formation. The potentiator is elaborated by bone marrow cells in limiting amounts requiring either high cell concentrations or an exogenous source of the activity for optimal colony growth.
除祖细胞和存在于WEHI-3细胞条件培养基中的集落刺激活性（CSA）外，最佳的小鼠巨核细胞集落形成还需要辅助骨髓细胞。这些辅助细胞具有粘附性，沉降速率为5.8毫米/小时，浮力密度为1.065 - 1.078克/立方厘米。当辐射剂量超过900拉德时，骨髓细胞的活性丧失。具有这些特征的骨髓细胞以及来自肺、骨干和腹腔渗出细胞的上清液，在增强小鼠骨髓培养物中巨核细胞集落发生率方面均有活性，其发生率高于WEHI-3细胞条件培养基中必需活性所刺激的水平。某些巨噬细胞系（J774、P388D1）能够产生这种活性。本研究证实，一种增强活性可增强CSA对巨核细胞集落形成的刺激作用。增强剂由骨髓细胞产生，但其量有限，为实现最佳集落生长，要么需要高细胞浓度，要么需要该活性的外源性来源。
