Grønhøj Larsen C, Ternowitz T, Grønhøj Larsen F, Zachariae C, Thestrup-Pedersen K
Department of Dermatology, Marselisborg Hospital, University of Aarhus, Denmark.
Contact Dermatitis. 1989 May;20(5):335-40. doi: 10.1111/j.1600-0536.1989.tb03169.x.
We have previously demonstrated that the epidermal content of the lymphocyte activating peptide ETAF/IL-1 and lymphocyte chemotactic factor (ELCF) increases during the development of a cell-mediated immune reaction, represented either by the tuberculin skin reaction or by a positive patch test in patients with contact allergy. The present study describes the epidermal content of these mediators during an irritant patch test reaction. The results show that ELCF, but not ETAF/IL-1, is significantly increased in the epidermis of an irritant patch test with 3% SLS or 5% croton oil, irrespective of the intensity of the clinical patch test reaction. We observed that simple occlusion of epidermis did not induce ELCF activity in healthy persons, whereas patients with previous or current eczema had a significant release of ELCF following such occlusion. These results seem to indicate that there exist important functional differences between allergic and irritant patch test reactions with respect to the presence of lymphocyte activating signals in epidermis.
我们之前已经证明，在以结核菌素皮肤反应或接触性过敏患者的阳性斑贴试验为代表的细胞介导免疫反应发展过程中，淋巴细胞激活肽ETAF/IL-1和淋巴细胞趋化因子（ELCF）的表皮含量会增加。本研究描述了在刺激性斑贴试验反应过程中这些介质的表皮含量。结果表明，在使用3%十二烷基硫酸钠（SLS）或5%巴豆油进行的刺激性斑贴试验中，无论临床斑贴试验反应的强度如何，表皮中的ELCF（而非ETAF/IL-1）都会显著增加。我们观察到，单纯对健康人的表皮进行封闭并不会诱导ELCF活性，而既往或当前患有湿疹的患者在进行这种封闭后会有显著的ELCF释放。这些结果似乎表明，在表皮中淋巴细胞激活信号的存在方面，过敏性和刺激性斑贴试验反应之间存在重要的功能差异。
