曼谷放线嗜皮菌44EHW的基因组序列草图揭示了具有不寻常丁基丙二酰辅酶A延伸单元的抗真菌化合物泰国菌素的生物合成途径。
The Draft Genome Sequence of Actinokineospora bangkokensis 44EHW Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units.
作者信息
Greule Anja, Intra Bungonsiri, Flemming Stephan, Rommel Marcel G E, Panbangred Watanalai, Bechthold Andreas
机构信息
Department of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs-University of Freiburg, Stefan-Meier-Straße 19, 79104 Freiburg, Germany.
Department of Biotechnology, Faculty of Science, Mahidol University, 272 Rama 6 Road, Bangkok 10400, Thailand.
出版信息
Molecules. 2016 Nov 23;21(11):1607. doi: 10.3390/molecules21111607.
We report the draft genome sequence of 44EHW, the producer of the antifungal polyene compounds, thailandins A and B. The sequence contains 7.45 Mb, 74.1% GC content and 35 putative gene clusters for the biosynthesis of secondary metabolites. There are three gene clusters encoding large polyketide synthases of type I. Annotation of the ORF functions and targeted gene disruption enabled us to identify the cluster for thailandin biosynthesis. We propose a plausible biosynthetic pathway for thailandin, where the unusual butylmalonyl-CoA extender unit is incorporated and results in an untypical side chain.
我们报道了抗真菌多烯化合物泰国菌素A和B的产生菌44EHW的基因组序列草图。该序列包含745万个碱基对，GC含量为74.1%，有35个假定的次生代谢物生物合成基因簇。存在三个编码I型大型聚酮合酶的基因簇。通过对开放阅读框功能的注释和靶向基因破坏，我们确定了泰国菌素生物合成的基因簇。我们提出了一个合理的泰国菌素生物合成途径，其中掺入了不寻常的丁基丙二酰辅酶A延伸单元，并产生了一个非典型的侧链。
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