Sakai Y, Kimura S, Yamashita T, Umetsu K, Suzuki T, Sendo F
Department of Parasitology, Yamagata University School of Medicine.
Microbiol Immunol. 1989;33(7):569-77. doi: 10.1111/j.1348-0421.1989.tb02007.x.
A sensitive thymocyte co-stimulator assay of IL-1 using a beta-D-galactoside specific lectin (allo A) obtained from the beetle (Allomyrina dichotoma) is reported here. Allo A stimulated [3H]thymidine uptake of mouse thymocytes in the presence of IL-1. The allo A assay was more sensitive than the PHA or PNA- thymocyte assay, especially at low doses of IL-1. Optimal conditions for the allo A assay were as follows: allo A, 2.5-5.0 micrograms/ml; whole thymocytes, 0.5-1.0 x 10(6) cells/well; incubation time, 72-96 hr. The assay is sensitive and convenient and can easily be performed in any laboratory.
本文报道了一种使用从独角仙（双叉犀金龟）获得的β-D-半乳糖苷特异性凝集素（相思豆凝集素）进行的白细胞介素-1敏感胸腺细胞共刺激测定法。在白细胞介素-1存在的情况下，相思豆凝集素刺激小鼠胸腺细胞摄取[3H]胸腺嘧啶核苷。相思豆凝集素测定法比植物血凝素或花生凝集素-胸腺细胞测定法更敏感，尤其是在低剂量白细胞介素-1时。相思豆凝集素测定法的最佳条件如下：相思豆凝集素，2.5 - 5.0微克/毫升；全胸腺细胞，0.5 - 1.0×10(6)个细胞/孔；孵育时间，72 - 96小时。该测定法灵敏且方便，任何实验室都能轻松进行。
