Kimura S, Umetsu K, Yamashita T, Suzuki T, Arai S, Sendo F
Immunopharmacology. 1987 Jun;13(3):181-8. doi: 10.1016/0162-3109(87)90056-7.
We examined the lymphocyte mitogenicity of a novel beta-D-galactoside-specific lectin from the beetle Allomyrina dichotoma, named allo A. Allo A was mitogenic to spleen cells of various strains of mice and rats and to human peripheral blood lymphocytes. When the selectivity of mitogenicity to T or B lymphocytes was examined with mouse spleen cells, allo A was selectively mitogenic to T-enriched spleen cells, which indicates that allo A is a T cell mitogen. Thymocytes from non-treated mice hardly responded to allo A, while those from cortisone-treated mice did to a great extent, indicating that allo A is mitogenic to mature thymocytes. The lymphocyte activation with allo A was inhibited by lactose, but not by melibiose, N-acetyl-D-glucosamine, or methyl-alpha-D-mannoside, which suggests that cell surface molecules containing beta-D-galactosyl residues are of importance in the structure of allo A receptors on the cell surface.
我们检测了一种从独角仙（Allomyrina dichotoma）中提取的新型β-D-半乳糖苷特异性凝集素（命名为allo A）的淋巴细胞促有丝分裂活性。Allo A对多种品系的小鼠和大鼠的脾细胞以及人外周血淋巴细胞具有促有丝分裂作用。当用小鼠脾细胞检测allo A对T淋巴细胞或B淋巴细胞的促有丝分裂选择性时，allo A对富含T细胞的脾细胞具有选择性促有丝分裂作用，这表明allo A是一种T细胞促有丝分裂原。未处理小鼠的胸腺细胞对allo A几乎没有反应，而经可的松处理小鼠的胸腺细胞则有很大反应，这表明allo A对成熟胸腺细胞具有促有丝分裂作用。乳糖可抑制allo A引起的淋巴细胞活化，但蜜二糖、N-乙酰-D-葡萄糖胺或α-D-甲基甘露糖苷则无此作用，这表明细胞表面含有β-D-半乳糖基残基的分子在细胞表面allo A受体结构中具有重要作用。
