Zhao Hongcui, Zhao Yue, Ren Yun, Li Min, Li Tianjie, Li Rong, Yu Yang, Qiao Jie
Ministry of Education Key Laboratory of Assisted Reproduction, and Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Center of Reproductive Medicine, Peking University Third Hospital, Beijing, People's Republic of China.
Ministry of Education Key Laboratory of Assisted Reproduction, and Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Center of Reproductive Medicine, Peking University Third Hospital, Beijing, People's Republic of China.
Fertil Steril. 2017 Feb;107(2):467-474.e5. doi: 10.1016/j.fertnstert.2016.10.039. Epub 2016 Nov 23.
To investigate PPARGC1A promoter methylation and mitochondria DNA (mtDNA) content in the leukocytes of women with polycystic ovary syndrome (PCOS) and analyze the relationship between these indices and metabolic risk for women with PCOS.
Cross-sectional study.
University hospital.
PATIENT(S): A total of 175 women with PCOS and 127 healthy controls.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Women with and without PCOS classified using the typical metabolic risk criteria of the National Cholesterol Education Program's Adult Treatment Panel III report (ATPIII), methylation of PPARGC1A promoter tested by methylation-specific polymerase chain reaction, and mtDNA content confirmed by quantitative polymerase chain reaction (PCR).
RESULT(S): PPARGC1A promoter methylation was specifically increased, but mtDNA content was specifically decreased in women with PCOS compared with the control women after adjustment for body mass index. Moreover, in women with PCOS who have increased metabolic risk, the differences in PPARGC1A promoter methylation and mitochondrial content were aggravated.
CONCLUSION(S): In conclusion, PPARGC1A promoter methylation and mitochondrial content were found to be potential biomarkers for the prediction of metabolic risk in women with PCOS.
研究多囊卵巢综合征（PCOS）女性白细胞中PPARGC1A启动子甲基化和线粒体DNA（mtDNA）含量，并分析这些指标与PCOS女性代谢风险之间的关系。
横断面研究。
大学医院。
共175例PCOS女性和127例健康对照者。
无。
根据美国国家胆固醇教育计划成人治疗小组第三次报告（ATPIII）的典型代谢风险标准对有无PCOS的女性进行分类，采用甲基化特异性聚合酶链反应检测PPARGC1A启动子甲基化，采用定量聚合酶链反应（PCR）确认mtDNA含量。
调整体重指数后，与对照女性相比，PCOS女性的PPARGC1A启动子甲基化特异性增加，但mtDNA含量特异性降低。此外，在代谢风险增加的PCOS女性中，PPARGC1A启动子甲基化和线粒体含量的差异更为明显。
总之，发现PPARGC1A启动子甲基化和线粒体含量是预测PCOS女性代谢风险的潜在生物标志物。
