Gu Riliang, Chen Fanjun, Long Lizhi, Cai Hongguang, Liu Zhigang, Yang Jiabo, Wang Lifeng, Li Huiyong, Li Junhui, Liu Wenxin, Mi Guohua, Zhang Fusuo, Yuan Lixing
Key Lab of Plant-Soil Interaction, MOE, Center for Resources, Environment and Food Security, College Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China; College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Key Lab of Plant-Soil Interaction, MOE, Center for Resources, Environment and Food Security, College Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
J Genet Genomics. 2016 Nov 20;43(11):663-672. doi: 10.1016/j.jgg.2016.11.002. Epub 2016 Nov 9.
Root system architecture (RSA) plays an important role in phosphorus (P) acquisition, but enhancing P use efficiency (PUE) in maize via genetic manipulation of RSA has not yet been reported. Here, using a maize recombinant inbred line (RIL) population, we investigated the genetic relationships between PUE and RSA, and developed P-efficient lines by selection of quantitative trait loci (QTLs) that coincide for both traits. In low-P (LP) fields, P uptake efficiency (PupE) was more closely correlated with PUE (r = 0.48-0.54), and RSA in hydroponics was significantly related to PupE (r = 0.25-0.30) but not to P utilization efficiency (PutE). QTL analysis detected a chromosome region where two QTLs for PUE, three for PupE and three for RSA were assigned into two QTL clusters, Cl-bin3.04a and Cl-bin3.04b. These QTLs had favorable effects from alleles derived from the large-rooted and high-PupE parent. Marker-assisted selection (MAS) identified nine advanced backcross-derived lines carrying Cl-bin3.04a or Cl-bin3.04b that displayed mean increases of 22%-26% in PUE in LP fields. Furthermore, a line L224 pyramiding Cl-bin3.04a and Cl-bin3.04b showed enhanced PupE, relying mainly on changes in root morphology, rather than root physiology, under both hydroponic and field conditions. These results highlight the physiological and genetic contributions of RSA to maize PupE, and provide a successful study case of developing P-efficient crops through QTL-based selection.
根系结构（RSA）在磷（P）吸收中起着重要作用，但尚未有通过对RSA进行基因操作来提高玉米磷利用效率（PUE）的报道。在此，我们利用一个玉米重组自交系（RIL）群体，研究了PUE与RSA之间的遗传关系，并通过选择与这两个性状重合的数量性状位点（QTL）来培育磷高效品系。在低磷（LP）田间，磷吸收效率（PupE）与PUE的相关性更强（r = 0.48 - 0.54），水培条件下的RSA与PupE显著相关（r = 0.25 - 0.30），但与磷利用效率（PutE）无关。QTL分析检测到一个染色体区域，其中两个PUE的QTL、三个PupE的QTL和三个RSA的QTL被归为两个QTL簇，即Cl-bin3.04a和Cl-bin3.04b。这些QTL从大根且高PupE的亲本衍生的等位基因中具有有利效应。标记辅助选择（MAS）鉴定出九个携带Cl-bin3.04a或Cl-bin3.04b的回交衍生高级品系，它们在LP田间的PUE平均提高了22% - 26%。此外，聚合了Cl-bin3.04a和Cl-bin3.04b的品系L224在水培和田间条件下均表现出增强的PupE，这主要依赖于根系形态而非根系生理的变化。这些结果突出了RSA对玉米PupE的生理和遗传贡献，并提供了一个通过基于QTL的选择培育磷高效作物的成功研究案例。
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