Yuan Yuanyuan, Gao Minggang, Zhang Mingxia, Zheng Honghua, Zhou Xiuwen, Guo Ying, Zhao Yan, Kong Fangmei, Li Sishen
State Key Laboratory of Crop Biology, National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural UniversityTai'an, China.
Jinan Academy of Agricultural ScienceJinan, China.
Front Plant Sci. 2017 Apr 24;8:614. doi: 10.3389/fpls.2017.00614. eCollection 2017.
Phosphorus (P) efficiency (PE), which comprises phosphorus uptake (PupE) and utilization efficiency (PutE), is considered as one of the most important factors for crop yield. In the present study, 11 seedling traits and 13 maturity traits related to wheat PE and morphology were investigated using a set of recombinant inbred lines (RILs) derived from the cross of "TN 18 × LM 6," under hydroponic culture trials and field trials at low P (LP) and normal P (NP) levels in two different years, respectively. The LP input reduced of biomass, yield and PupE traits, but increased PutE traits. A total of 163 QTLs for seedling and maturity traits under different P levels and their AV, and 15 QTLs for relative traits were detected on 21 chromosomes. Of these, 49 and 63 QTLs for were detected specially in LP and NP treatments, respectively. We found 11 relatively high-frequency QTLs (RHF-QTLs) and four important QTL clusters, which may be the potential targets for marker-assisted selection (MAS) in wheat breeding programs for PE. Favorable relationships for breeding programs were found in the four important QTL clusters, which allow the possibility of improving the morphological traits and PutE simultaneously. A total of 29 markers which associated with 51 QTLs were found highly homologous with EST sequences, which suggested that they were potential functional loci. We suggested that the four biomass traits (SDW, RDW, TDW, and RSDW), five yield traits (SN, PH, TGW, GWP, and StWP) and two relative traits (Rstwp and Rgwp) can be considered as the primary indexes for the evaluation of PE for they are easy to identify on a large-scale.
磷（P）效率（PE）包括磷吸收效率（PupE）和利用效率（PutE），被认为是影响作物产量的最重要因素之一。在本研究中，利用一组由“TN 18×LM 6”杂交衍生的重组自交系（RILs），分别在两年的水培试验和田间试验中，于低磷（LP）和正常磷（NP）水平下，对11个与小麦磷效率和形态相关的苗期性状以及13个成熟期性状进行了研究。低磷投入降低了生物量、产量和磷吸收效率性状，但提高了磷利用效率性状。在21条染色体上共检测到163个不同磷水平下苗期和成熟期性状及其加性效应值（AV）的QTL，以及15个相对性状的QTL。其中，分别在低磷和正常磷处理中特异检测到49个和63个QTL。我们发现了11个相对高频QTL（RHF-QTL）和4个重要的QTL簇，它们可能是小麦磷效率育种计划中标记辅助选择（MAS）的潜在目标。在这4个重要的QTL簇中发现了有利于育种计划的关系，这使得同时改善形态性状和磷利用效率成为可能。共发现29个与51个QTL相关的标记与EST序列高度同源，这表明它们是潜在的功能位点。我们建议，四个生物量性状（地上部干重、根干重、总干重和根冠比）、五个产量性状（穗数、株高、千粒重、地上部生物量和茎生物量）和两个相对性状（相对茎生物量和相对地上部生物量）可被视为评估磷效率的主要指标，因为它们易于大规模鉴定。
