Wu Yukun, Wu Jing, Cao Jing, Zhang Yajie, Xu Zhe, Qin Xiuyi, Wang Wei, Yuan Zhi
Key Laboratory of Functional Polymer Materials of Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China.
Key Laboratory of Functional Polymer Materials of Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China; Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, Tianjin 300071, China.
Eur J Pharm Biopharm. 2017 Mar;112:148-154. doi: 10.1016/j.ejpb.2016.11.020. Epub 2016 Nov 23.
Chitosan is one of the most important and commonly used natural polysaccharides in drug delivery for its biocompatible and biodegradable properties. However, poor blood circulation of the chitosan nanoparticles due to their cationic nature is one of the major bottlenecks of chitosan-based drug delivery systems. To address this problem, a versatile platform based on poly(acrylic acid) (PAA) coated ionically cross-linked chitosan/tripolyphosphate nanoparticles (CTS/TPP-PAA NPs), is reported. The zeta potentials of CTS/TPP and CTS/TPP-PAA NPs are approximately 33mV and -25mV, respectively. CTS/TPP NPs quickly aggregate in PBS (phosphate buffered saline) and DMEM (Dulbecco's modified Eagle's medium). Conversely, CTS/TPP-PAA NPs exhibit excellent colloidal stability in plasma solution for more than 24h. The PAA coating also endows CTS/TPP-PAA NPs with decreased protein adsorption capacity and improved buffering capacity. More importantly, the residual carboxyl and amino groups on CTS/TPP-PAA NPs provide abundant reactive sites for further functional modifications. Therefore, the CTS/TPP-PAA NPs reported here may be useful as an alternative drug delivery system.
壳聚糖因其生物相容性和可生物降解性，是药物递送中最重要且常用的天然多糖之一。然而，壳聚糖纳米颗粒因其阳离子性质导致血液循环不佳，这是基于壳聚糖的药物递送系统的主要瓶颈之一。为解决这一问题，本文报道了一种基于聚（丙烯酸）（PAA）包覆的离子交联壳聚糖/三聚磷酸钠纳米颗粒（CTS/TPP-PAA NPs）的多功能平台。CTS/TPP和CTS/TPP-PAA NPs的zeta电位分别约为33mV和-25mV。CTS/TPP NPs在PBS（磷酸盐缓冲盐水）和DMEM（杜氏改良伊格尔培养基）中迅速聚集。相反，CTS/TPP-PAA NPs在血浆溶液中表现出超过24小时的优异胶体稳定性。PAA涂层还使CTS/TPP-PAA NPs的蛋白质吸附能力降低，缓冲能力提高。更重要的是，CTS/TPP-PAA NPs上残留的羧基和氨基为进一步的功能修饰提供了丰富的反应位点。因此，本文报道的CTS/TPP-PAA NPs可能作为一种替代的药物递送系统。
