Laboratory of Mesoscopic Chemistry and Department of Polymer Science and Engineering, College of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
Macromol Biosci. 2009 Dec 8;9(12):1272-80. doi: 10.1002/mabi.200900245.
Chitosan-poly(acrylic acid)-gold (CS-PAA-Au) hybrid hollow nanospheres were prepared by reducing gold salts to gold nanoparticles in chitosan-acrylic acid (CS-AA) aqueous solution, followed by polymerization of AA monomer and selectively crosslinking chitosan at the end of polymerization. The payload of Au nanoparticles within the CS-PAA hollow nanospheres could be controlled by varying the addition amount of gold salts in the reaction system. Transmission electron microscopy (TEM) revealed that multi-crystal gold nanoparticles were encapsulated inside the CS-PAA nanospheres. TEM and scanning electron microscopy (SEM) indicated that these hybrid nanospheres had a hollow structure. Energy-dispersive X-ray (EDX) spectroscopy confirmed the existence of gold nanoparticles inside the CS-PAA-Au hybrid nanospheres. These hollow CS-PAA-Au hybrid nanospheres showed a typical surface plasmon resonance (SPR) band of gold nanoparticles around 526 nm, and offered excellent stability at different temperatures and in acidic media. Also, no inhibition of cell proliferation and no cytotoxic effects were observed in the presence of CS-PAA-Au hybrid nanospheres, which renders them good candidates for potential application in biomedical fields.
壳聚糖-聚丙烯酸-金（CS-PAA-Au）杂化中空纳米球是通过在壳聚糖-丙烯酸（CS-AA）水溶液中将金盐还原为金纳米粒子，然后聚合 AA 单体，并在聚合结束时选择性地交联壳聚糖来制备的。通过改变反应体系中金盐的添加量，可以控制 CS-PAA 中空纳米球内的 Au 纳米粒子的载药量。透射电子显微镜（TEM）显示多晶金纳米粒子被包裹在 CS-PAA 纳米球内。TEM 和扫描电子显微镜（SEM）表明这些杂化纳米球具有中空结构。能谱（EDX）证实了 CS-PAA-Au 杂化纳米球内存在金纳米粒子。这些中空 CS-PAA-Au 杂化纳米球在 526nm 左右表现出典型的金纳米粒子表面等离子体共振（SPR）带，并且在不同温度和酸性介质中表现出优异的稳定性。此外，在存在 CS-PAA-Au 杂化纳米球的情况下，没有观察到细胞增殖的抑制和细胞毒性作用，这使得它们成为生物医学领域潜在应用的良好候选材料。
