Zhang Ruizhong, Lin Zefeng, Lui Vincent C H, Wong Kenneth K Y, Tam Paul K H, Lee Puiyan, Lok Chun Nam, Lamb Jonathan R, Chen Yan, Xia Huimin
Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.
Department of Surgery, The University of Hong Kong, Hong Kong, SAR, China.
Nanomedicine. 2017 Apr;13(3):1041-1050. doi: 10.1016/j.nano.2016.11.013. Epub 2016 Nov 25.
Biliary atresia (BA) is a neonatal biliary system disease closely associated with viral infection and bile duct inflammation. Silver nanoparticles (AgNps) have previously revealed antiviral and anti-inflammatory properties. In this study, we have investigated the effects of AgNps in the treatment of the Rhesus rotavirus inoculation induced BA in mice. The morphology, liver histopathology, clinical biochemistry examination, and inflammatory cells were analyzed in BA mice. Results indicated that AgNps could significantly increase the survival rate of BA mice, and reduce jaundice and weight lost and the liver enzymes and bilirubin metabolism clinical parameters were close to the normal levels. Diminished numbers of NK cells were observed by flow cytometry analysis and immunohistochemical staining. Furthermore, the viral load was reduced and transcripts for TGF-β mRNA were augmented after AgNps treatment. Collectively, our results suggest that AgNps treatment has beneficial effects on the BA mouse model partially through upregulation of TGF-β.
胆道闭锁（BA）是一种与病毒感染和胆管炎症密切相关的新生儿胆道系统疾病。银纳米颗粒（AgNps）此前已显示出抗病毒和抗炎特性。在本研究中，我们研究了AgNps对恒河猴轮状病毒接种诱导的小鼠BA的治疗效果。对BA小鼠的形态、肝脏组织病理学、临床生化检查和炎性细胞进行了分析。结果表明，AgNps可显著提高BA小鼠的存活率，减轻黄疸和体重减轻，肝脏酶和胆红素代谢临床参数接近正常水平。通过流式细胞术分析和免疫组织化学染色观察到NK细胞数量减少。此外，AgNps治疗后病毒载量降低，TGF-β mRNA转录物增加。总体而言，我们的结果表明，AgNps治疗对BA小鼠模型具有有益作用，部分原因是通过上调TGF-β实现的。
