中国江西犬类蜱传病原体的首次分子检测。

First molecular detection of tick-borne pathogens in dogs from Jiangxi, China.

作者信息

Zheng Weiqing, Liu Mingming, Moumouni Paul Franck Adjou, Liu Xiaoqing, Efstratiou Artemis, Liu Zhanbin, Liu Yangqing, Tao Huiying, Guo Huanping, Wang Guanbo, Gao Yang, Li Zifen, Ringo Aaron Edmund, Jirapattharasate Charoonluk, Chen Haiying, Xuan Xuenan

机构信息

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.

出版信息

J Vet Med Sci. 2017 Feb 4;79(2):248-254. doi: 10.1292/jvms.16-0484. Epub 2016 Nov 26.

In this study, blood samples obtained from 162 dogs in Jiangxi, China, were employed in molecular screening of canine tick-borne pathogens by PCR and sequencing. Babesia spp. gene fragment was detected in 12 (7.41%) dogs. All samples were negative for Hepatozoon spp., Ehrlichia canis, Coxiella spp., Borrelia spp., Rickettsia spp. and Anaplasma platys. Species-specific PCR analysis further confirmed that 8 (4.94%) and 4 (2.47%) dogs were infected by Babesia canis vogeli and Babesia gibsoni, respectively. Based on our analyses, Babesia spp. infection in Jiangxi appeared not related to age, gender, breed, usage, activity and health status or tick infestation history of the dogs. This is the first molecular report of Babesia canis vogeli and Babesia gibsoni in dogs from Jiangxi, China.

在本研究中，从中国江西的162只犬采集血液样本，采用聚合酶链反应（PCR）和测序技术对犬蜱传病原体进行分子筛查。在12只（7.41%）犬中检测到巴贝斯虫属基因片段。所有样本的肝簇虫属、犬埃立克体、柯克斯体属、疏螺旋体属、立克次体属和扁平无形体均为阴性。种特异性PCR分析进一步证实，分别有8只（4.94%）和4只（2.47%）犬感染了伏氏犬巴贝斯虫和吉氏巴贝斯虫。根据我们的分析，江西犬的巴贝斯虫属感染似乎与犬的年龄、性别、品种、用途、活动和健康状况或蜱虫感染史无关。这是中国江西犬中伏氏犬巴贝斯虫和吉氏巴贝斯虫的首次分子报告。