Plachý J, Vilhelmová M, Schulmannová J, Karakoz I
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Folia Biol (Praha). 1989;35(3):121-36.
Using Prague recombinant congenic lines of chickens, we found that neonatal thymectomy led either to a marked suppression (CC donors) or to a transient increase (CB donors) of GvH response against the B-F/L + B-G and B-F/L disparate congenic embryos. Similarly, preimmunization with the B (MHC) different blood led either to the suppression or increase of GvH reactivity of the CC and CB line donors depending on the antigenic difference (B-F/L + B-G, B-F/L, B-G) between the blood used for preimmunization, the immunized donor for GvH splenomegaly assay and the recipient embryos. In all cases, the suppression of GvH response of the CC line donors is accompanied with a marked increase in GvH reactivity against syngeneic and B-G 12 disparate embryos, suggesting an autoimmune state. Furthermore, the CC line chickens suffering from GvHD had lower GvH reactivity against the B (MHC) different embryos and a significantly raised reactivity against syngeneic embryos in comparison with controls. Chickens of the same group recovering from GvHD had an extremely high alloreactivity and only slightly or no increased GvH reactivity against syngeneic embryos.
利用布拉格重组近交系鸡，我们发现新生期胸腺切除要么导致对B - F/L + B - G和B - F/L不相合近交胚胎的移植物抗宿主反应（GvH）显著抑制（CC供体），要么导致短暂增强（CB供体）。同样，用B（主要组织相容性复合体）不同的血液进行预免疫，根据预免疫所用血液、用于GvH脾肿大测定的免疫供体和受体胚胎之间的抗原差异（B - F/L + B - G、B - F/L、B - G），CC和CB系供体的GvH反应性会出现抑制或增强。在所有情况下，CC系供体的GvH反应抑制伴随着对同基因和B - G 12不相合胚胎的GvH反应性显著增强，提示处于自身免疫状态。此外，与对照组相比，患有移植物抗宿主病（GvHD）的CC系鸡对B（主要组织相容性复合体）不同胚胎的GvH反应性较低，对同基因胚胎的反应性显著升高。从GvHD恢复的同一组鸡具有极高的同种异体反应性，对同基因胚胎的GvH反应性仅略有增加或没有增加。
