Shinomiya Kazufusa, Umezawa Motoki, Seki Manami, Nitta Jun, Zaima Kazumasa, Harikai Naoki, Ito Yoichiro
School of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi-shi, Chiba 274-8555, Japan.
Laboratory of Bioseparation Technology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 8N230, Bethesda, MD 20892-1762, USA.
Separations. 2016 Dec;3(4). doi: 10.3390/separations3040029. Epub 2016 Sep 22.
1 BACKGROUND: Countercurrent chromatography (CCC) is liquid-liquid partition chromatography without using a solid support matrix. This technique requires further improvement of partition efficiency and shortening theseparation time.
2 METHODS: The locular multilayer coils modified with and without mixer glass beads were developed for the separation of proteins and 4-methylumbelliferyl (MU) sugar derivatives using the small-scale cross-axis coil planet centrifuge.
3 RESULTS: Proteins were well separated from each other and the separation was improved at a low flow rate of the mobile phase. On the other hand, 4-MU sugar derivatives were sufficiently resolved with short separation time at a highflow rate of the mobile phase under satisfactory stationary phase retention.
4 CONCLUSION: Effective separations were achieved using the locular multilayer coil for proteins with aqueous-aqueous polymer phase systems and for 4-MU sugar derivatives with organic-aqueous two-phase solvent systems by inserting a glass bead into each locule.
1 背景：逆流色谱法（CCC）是一种不使用固体支持基质的液-液分配色谱法。该技术需要进一步提高分配效率并缩短分离时间。
2 方法：使用小型交叉轴线圈行星离心机，开发了带有和不带有混合玻璃珠的小室多层线圈，用于分离蛋白质和4-甲基伞形酮（MU）糖衍生物。
3 结果：蛋白质之间得到了很好的分离，并且在流动相流速较低时分离效果有所改善。另一方面，在令人满意的固定相保留率下，4-MU糖衍生物在流动相流速较高时能在短时间内得到充分分离。
4 结论：通过在每个小室中插入玻璃珠，使用小室多层线圈对水-水聚合物相系统中的蛋白质和有机-水两相溶剂系统中的4-MU糖衍生物实现了有效分离。
