Nickerson S C, Baker P A, Trinidad P
Mastitis Research Laboratory, Louisiana State University Agricultural Center, Homer 71040.
J Dairy Sci. 1989 Jul;72(7):1764-73. doi: 10.3168/jds.S0022-0302(89)79293-6.
One day after drying off, miniosmotic pumps releasing interleukin-2 were implanted into teat cisterns of six Jersey cows. Each cow had two quarters with (sensitized) and without (unsensitized) chronic Staphylococcus aureus infection. At 1 and 3 wk after implantation, three cows were sacrificed and mammary tissues processed to examine the cytological response to interleukin-2. Concentrations of lymphoid cells were determined in tissues lining teat and gland cistern linings, and plasma cells were characterized by Ig class. For total lymphoid cell and IgG1 and IgG2 plasma cell concentrations, sensitized placebo quarters and unsensitized interleukin-2 quarters were similar, indicating interleukin-2 stimulated a cellular immune response equal to that of quarters infected with S. aureus. Greatest response was observed in sensitized interleukin-2 quarters, suggesting that the cytokine was more effective in quarters with prior exposure to S. aureus antigens. Greater numbers of lymphocytes and macrophages were found in secretions of interleukin-2 quarters than in secretion of control quarters. Local interleukin-2 immunostimulation may supplement immunization procedures and enhance protection against natural S. aureus challenge.
干燥一天后，将释放白细胞介素-2的微量渗透泵植入6头泽西奶牛的乳头池。每头奶牛有两个乳腺区，一个有（致敏）慢性金黄色葡萄球菌感染，另一个没有（未致敏）慢性金黄色葡萄球菌感染。植入后1周和3周，处死3头奶牛并对乳腺组织进行处理，以检查对白细胞介素-2的细胞学反应。测定乳头内衬组织和腺池内衬组织中的淋巴细胞浓度，并根据免疫球蛋白类别对浆细胞进行鉴定。对于总淋巴细胞以及IgG1和IgG2浆细胞浓度，致敏的安慰剂乳腺区和未致敏的白细胞介素-2乳腺区相似，这表明白细胞介素-2刺激产生的细胞免疫反应与感染金黄色葡萄球菌的乳腺区相当。在致敏的白细胞介素-2乳腺区观察到最大反应，这表明该细胞因子在先前接触过金黄色葡萄球菌抗原的乳腺区更有效。白细胞介素-2乳腺区分泌物中的淋巴细胞和巨噬细胞数量多于对照乳腺区的分泌物。局部白细胞介素-2免疫刺激可能会补充免疫程序，并增强对自然金黄色葡萄球菌攻击的保护作用。
