Bastos Clarissa R, Gazal Marta, Quevedo Luciana de A, Costa Joice Luisa, Wiener Carolina D, Jansen Karen, de Mola Christian Loret, Oses Jean P, Souza Luciano D M, Portela Luiz Valmor, Pinheiro Ricardo T, da Silva Ricardo A, Lara Diogo R, Ghisleni Gabriele
Programa de Pós-Graduação em Saúde e Comportamento, Universidade Católica de Pelotas, Brazil.
Biologia Celular e Molecular, Pontifícia Universidade Católica do Rio Grande do Sul, Brazil.
J Psychiatr Res. 2017 Mar;86:34-38. doi: 10.1016/j.jpsychires.2016.11.009. Epub 2016 Nov 18.
Approximately one million people commit suicide every year, being suicide attempts and ideation even more common. Changes in stress response and activation of the immune system have been associated with suicide risk. Here we investigated the interaction between immune system and HPA axis alterations in the suicide risk, looking for the influence of rs110402 CRHR1 SNP in the IL-1β levels according to suicide ideation and attempt. This study evaluated 171 subjects of which 15 had suicidal ideation, 20 had suicide attempt and 136 were controls. Genotyping was performed by real-time PCR and IL-1β levels were measured by ELISA. Our data showed that for each point increase in IL-1β levels the risk of suicide attempt increased 5% [relative risk = 1.05 (95% CI: 1.0-1.10)]. After sample stratification by rs110402 SNP genotypes, we observed that in subjects carrying the A allele the risk raised to 15% [relative risk = 1.15 (95% CI: 1.03-1.28)], suggesting an apparent effect modification. Thus, this study showed that alterations in CRHR1 gene were associated with higher levels of IL-1β, and increased risk for suicide, reinforcing the importance of multifactorial interactions of biological markers for psychiatric disorders.
每年约有100万人自杀，自杀未遂和自杀念头更为常见。应激反应的变化和免疫系统的激活与自杀风险有关。在此，我们研究了免疫系统与HPA轴改变在自杀风险中的相互作用，根据自杀念头和自杀未遂情况寻找rs110402 CRHR1单核苷酸多态性对白细胞介素-1β水平的影响。本研究评估了171名受试者，其中15人有自杀念头，20人有自杀未遂经历，136人作为对照。通过实时聚合酶链反应进行基因分型，通过酶联免疫吸附测定法测量白细胞介素-1β水平。我们的数据显示，白细胞介素-1β水平每升高一个点，自杀未遂风险增加5%[相对风险=1.05(95%置信区间：1.0-1.10)]。按rs110402单核苷酸多态性基因型对样本进行分层后，我们观察到携带A等位基因的受试者风险升至15%[相对风险=1.15(95%置信区间：1.03-1.28)]，提示存在明显的效应修饰。因此，本研究表明CRHR1基因的改变与白细胞介素-1β水平升高及自杀风险增加有关，强化了生物标志物多因素相互作用对精神疾病的重要性。
