Moon H B, Severinson E, Heusser C, Johansson S G, Möller G, Persson U
Department of Clinical Immunology, Karolinska Hospital, Stockholm, Sweden.
Scand J Immunol. 1989 Sep;30(3):355-61. doi: 10.1111/j.1365-3083.1989.tb01221.x.
Mouse interleukin 4 (IL-4) has been shown to act on B cells as an induction factor for Ig class switch. We studied the characteristics of IL-4-regulated Ig isotype production in lipopolysaccharide (LPS)-stimulated splenic B-cell cultures with emphasis on the comparison between the IgG1 and IgE responses. The results show that the kinetics for the appearance of IgG1 and IgE isotypes are similar, but that the dose of IL-4 required for the induction of an IgE response is 3-10 times higher than that for an IgG1 response. No requirement for T cells was found for the induction of either isotype. Pre-incubation of cells for 24 h with IL-4 alone was sufficient to induce an IgG1 response when cells were recultured with LPS from days 1 to 6. However, the simultaneous presence of both IL-4 and LPS for at least 24 h was required for a detectable IgE response. For an optimal IgE response, IL-4 needed to be present for more than 72 h in LPS-activated cultures. The possible reasons for the different regulation of IgG1 and IgE responses are discussed.
小鼠白细胞介素4（IL-4）已被证明作为Ig类别转换的诱导因子作用于B细胞。我们研究了脂多糖（LPS）刺激的脾B细胞培养物中IL-4调节的Ig同种型产生的特征，重点是比较IgG1和IgE反应。结果表明，IgG1和IgE同种型出现的动力学相似，但诱导IgE反应所需的IL-4剂量比诱导IgG1反应所需的剂量高3至10倍。未发现诱导任何一种同种型需要T细胞。当细胞在第1天至第6天用LPS重新培养时，单独用IL-4预孵育细胞24小时足以诱导IgG1反应。然而，可检测到的IgE反应需要IL-4和LPS同时存在至少24小时。为了获得最佳的IgE反应，在LPS激活的培养物中IL-4需要存在超过72小时。讨论了IgG1和IgE反应不同调节的可能原因。
