Navarro-Guillén Carmen, Yúfera Manuel, Engrola Sofia
Instituto de Ciencias Marinas de Andalucía (ICMAN-CSIC), Apartado Oficial, Cádiz, Puerto Real 11519, Spain
Instituto de Ciencias Marinas de Andalucía (ICMAN-CSIC), Apartado Oficial, Cádiz, Puerto Real 11519, Spain.
Biol Open. 2017 Jan 15;6(1):77-82. doi: 10.1242/bio.021642.
Fish hatcheries must adapt larval feeding protocols to feeding behavior and metabolism patterns to obtain more efficient feed utilization. Fish larvae exhibit daily ingesting rhythms rather than ingesting food continuously throughout the day. The aim of this study was to determine the daily patterns of feed intake, protein digestibility, protein retention and catabolism in Senegalese sole post-larvae (Solea senegalensis; 33 days post-hatching) using C-labeled Artemia protein and incubation in metabolic chambers. Sole post-larvae were fed at 09:00, 15:00, 21:00, 03:00 and 09:00+1 day; and those fed at 09:00, 21:00, 03:00 and 09:00+1 day showed significantly higher feed intake than post-larvae fed at 15:00 h (P=0.000). Digestibility and evacuation rate of ingested protein did not change during the whole cycle (P=0.114); however, post-larvae fed at 21:00 and 03:00 h showed the significantly highest protein retention efficiency and lowest catabolism (P=0.002). Therefore, results confirm the existence of daily rhythmicity in feeding activity and in the utilization of the ingested nutrients in Senegalese sole post-larvae.
鱼类孵化场必须根据幼鱼的摄食行为和代谢模式调整幼体投喂方案，以提高饲料利用率。鱼类幼体呈现出每日摄食节律，而非全天持续摄食。本研究的目的是使用碳标记的卤虫蛋白并在代谢室中进行培养，来确定塞内加尔鳎幼鱼（孵化后33天）的每日摄食模式、蛋白质消化率、蛋白质保留率和分解代谢情况。塞内加尔鳎幼鱼分别在09:00、15:00、21:00、03:00和09:00 + 1天进行投喂；与在15:00投喂的幼鱼相比，在09:00、21:00、03:00和09:00 + 1天投喂的幼鱼摄食量显著更高（P = 0.000）。在整个周期内，摄入蛋白质的消化率和排空率没有变化（P = 0.114）；然而，在21:00和03:00投喂的幼鱼蛋白质保留效率显著最高，分解代谢最低（P = 0.002）。因此，结果证实了塞内加尔鳎幼鱼的摄食活动以及摄入营养物质利用方面存在每日节律性。
