Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional Agroalimentario CeiA3, Universidad de Córdoba, Campus de Rabanales, Edificio Severo Ochoa, E-14071 Córdoba, Spain.
Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, 29071, Málaga, Spain.
Fish Shellfish Immunol. 2018 Jun;77:350-363. doi: 10.1016/j.fsi.2018.04.018. Epub 2018 Apr 7.
Senegalese sole (Solea senegalensis) has been proposed as a high-potential species for aquaculture diversification in Southern Europe. It has been demonstrated that a proper feeding regimen during the first life stages influences larval growth and survival, as well as fry and juvenile quality. The bacterial strain Shewanella putrefaciens Pdp11 (SpPdp11) has shown very good probiotic properties in Senegalese sole, but information is scarce about its effect in the earliest stages of sole development. Thus, the aim of this study was to investigate the effect of SpPdp11, bioencapsulated in live diet, administered during metamorphosis (10-21 dph) or from the first exogenous feeding of Senegalese sole (2-21 dph). To evaluate the persistence of the probiotic effect, we sampled sole specimens from metamorphosis until the end of weaning (from 23 to 73 dph). This study demonstrated that probiotic administration from the first exogenous feeding produced beneficial effects on Senegalese sole larval development, given that specimens fed this diet exhibited higher and less dispersed weight, as well as increases in both total protein concentration and alkaline phosphatase activity, and in non-specific immune response. Moreover, real-time PCR documented changes in the expression of a set of genes involved in central metabolic functions including genes related to growth, genes coding for proteases (including several digestive enzymes), and genes implicated in the response to stress and in immunity. Overall, these results support the application of SpPdp11 in the first life stages of S. senegalensis as an effective tool with the clear potential to benefit sole aquaculture.
塞内加尔鳎（Solea senegalensis）已被提议作为欧洲南部水产养殖多样化的一个高潜力物种。已经证明，在生命早期阶段进行适当的饲养方案会影响幼虫的生长和存活，以及幼鱼和幼体的质量。细菌菌株希瓦氏菌（Shewanella putrefaciens）Pdp11（SpPdp11）在塞内加尔鳎中表现出非常好的益生菌特性，但关于其在鳎发育的最早阶段的影响的信息很少。因此，本研究的目的是研究 SpPdp11 的效果，SpPdp11 被包封在活饵料中，在变态期（10-21 dph）或从塞内加尔鳎的第一次外源性摄食开始（2-21 dph）进行给药。为了评估益生菌效果的持久性，我们从变态期开始对鳎标本进行采样，直到断奶结束（从 23 到 73 dph）。本研究表明，从第一次外源性摄食开始给予益生菌对塞内加尔鳎幼虫发育有有益的影响，因为喂食这种饲料的标本表现出更高和更少分散的体重，以及总蛋白浓度和碱性磷酸酶活性的增加，以及非特异性免疫反应的增加。此外，实时 PCR 记录了一组涉及中央代谢功能的基因的表达变化，包括与生长相关的基因、编码蛋白酶的基因（包括几种消化酶）以及与应激和免疫反应相关的基因。总的来说，这些结果支持将 SpPdp11 应用于 S. senegalensis 的生命早期阶段，作为一种有效的工具，具有明显的有益于鳎水产养殖的潜力。
