Park Hyunsoo, Lee Myunghwa, Kim Dae Woon, Hong Seo Yoo, Lee Hojung
Department of Obstetrics and Gynecology, Eulji General Hospital, Eulji University School of Medicine, Seoul, Korea.
Department of Pathology, Eulji General Hospital, Eulji University School of Medicine, Seoul, Korea.
Obstet Gynecol Sci. 2016 Nov;59(6):470-478. doi: 10.5468/ogs.2016.59.6.470. Epub 2016 Nov 15.
Glycogen synthase kinase 3β (GSK3β) is a pluripotent protein kinase involved in the development of cancers through regulation of numerous oncogenic molecules. Cyclin D1, an important regulator of G1 to S phase transition in various cells, is one of target proteins that GSK3β regulate. Our objective was to assess the expression of GSK3β and cyclin D1 in cervical neoplasm of different histologic grades and to identify their correlation in cervical carcinogenesis.
Immunohistochemical analysis of GSK3β and cyclin D1 was performed in a total of 137 patients with 12 normal, 62 cervical intraepithelial neoplasia (CIN) (31 CIN1 and 31 CIN3) and 63 invasive cancers including 56 squamous cell carcinomas and 7 adenocarcinomas.
The expression of GSK3β increased in parallel with the lesion grade, while that of cyclin D1 decreased with severity of the lesion (<0.001). There was a significant inverse correlation between GSK3β and cyclin D1 expression in overall cervical neoplasia (Φ=-0.413, <0.001). GSK3β expression was higher in squamous cell carcinoma than in adenocarcinoma (=0.049).
These results suggest that the expressional increase in GSK3β plays a role in cervical carcinogenesis and has inverse correlation with cyclin D1 expression in this process. In addition, GSK3β expression appears to be associated with the histologic type of cervical cancer, especially squamous cell carcinoma.
糖原合酶激酶3β（GSK3β）是一种多能蛋白激酶，通过调节众多致癌分子参与癌症的发生发展。细胞周期蛋白D1是各种细胞中G1期到S期转换的重要调节因子，是GSK3β调节的靶蛋白之一。我们的目的是评估不同组织学分级的宫颈肿瘤中GSK3β和细胞周期蛋白D1的表达，并确定它们在宫颈癌发生中的相关性。
对137例患者进行了GSK3β和细胞周期蛋白D1的免疫组织化学分析，其中包括12例正常宫颈组织、62例宫颈上皮内瘤变（CIN）（31例CIN1和31例CIN3）以及63例浸润癌，包括56例鳞状细胞癌和7例腺癌。
GSK3β的表达随病变分级升高而增加，而细胞周期蛋白D1的表达随病变严重程度降低（<0.001）。在总体宫颈肿瘤中，GSK3β和细胞周期蛋白D1的表达呈显著负相关（Φ=-0.413，<0.001）。鳞状细胞癌中GSK3β的表达高于腺癌（=0.049）。
这些结果表明，GSK3β表达增加在宫颈癌发生中起作用，并且在此过程中与细胞周期蛋白D1表达呈负相关。此外，GSK3β表达似乎与宫颈癌的组织学类型有关，尤其是鳞状细胞癌。
