Department of Histopathology, King Edward Memorial Hospital, Perth, WA, Australia.
Histopathology. 2012 Dec;61(6):1125-33. doi: 10.1111/j.1365-2559.2012.04326.x.
Immunohistochemistry is helpful in distinguishing cervical neoplastic lesions from their histological mimics, but has contributed less towards the sometimes problematic distinction of in-situ and superficially invasive tumours. Epithelial-mesenchymal transition (EMT) may be a mechanism of invasion in cervical neoplasia and expression of EMT-associated proteins could prove useful in this diagnostic setting.
Immunohistochemical expression of cyclin D1, E-cadherin and beta-catenin was assessed in 22 biopsy specimens from FIGO Stage IA cervical squamous carcinomas, all of which also included foci of cervical intraepithelial neoplasia (CIN) 3, nine biopsies of CIN 3 adjacent to carcinoma, and 10 cases of CIN 3 only. Most invasive tumour cells expressed cyclin D1 and showed a reduction in E-cadherin and beta-catenin staining. Nuclear beta-catenin expression was not observed. Cyclin D1 staining was reduced or showed altered distribution in most cases of CIN 3, while adhesion protein expression generally was preserved. However, altered protein expression similar to that of invasion was seen in some CIN lesions.
Most superficially invasive cervical squamous carcinomas show immunophenotypical changes consistent with EMT. These alterations, particularly cyclin D1 expression, may be useful diagnostically. Similar changes in CIN 3 lesions may indicate the acquisition of increased invasive potential.
免疫组织化学有助于区分宫颈癌前病变与其组织学模拟物，但在区分原位和表浅浸润性肿瘤方面的作用较小。上皮-间充质转化（EMT）可能是宫颈癌发生侵袭的一种机制，EMT 相关蛋白的表达在这种诊断环境中可能很有用。
评估了 22 例 FIGO 分期 IA 宫颈鳞癌活检标本中 cyclin D1、E-钙黏蛋白和β-连环蛋白的免疫组织化学表达，所有标本均包括宫颈上皮内瘤变（CIN）3 病灶，9 例癌旁 CIN 3 活检，和 10 例 CIN 3 仅。大多数侵袭性肿瘤细胞表达 cyclin D1，并显示 E-钙黏蛋白和β-连环蛋白染色减少。未观察到核β-连环蛋白表达。大多数 CIN 3 病例的 cyclin D1 染色减少或分布改变，而粘附蛋白表达通常保留。然而，一些 CIN 病变中也出现了类似侵袭的蛋白表达改变。
大多数表浅浸润性宫颈鳞癌显示与 EMT 一致的免疫表型改变。这些改变，特别是 cyclin D1 表达，可能具有诊断价值。CIN 3 病变中的类似改变可能表明侵袭潜能增加。
