Lees William D, Shepherd Adrian J
Department of Biological Sciences and Institute of Structural and Molecular Biology, Birkbeck, University of London, Malet Street, London, WC1E 7HX, UK.
Methods Mol Biol. 2017;1526:257-270. doi: 10.1007/978-1-4939-6613-4_15.
Next-generation sequencing is making it possible to study the antibody repertoire of an organism in unprecedented detail, and, by so doing, to characterize its behavior in the response to infection and in pathological conditions such as autoimmunity and cancer. The polymorphic nature of the repertoire poses unique challenges that rule out the use of many commonly used NGS methods and require tradeoffs to be made when considering experimental design.We outline the main contexts in which antibody repertoire analysis has been used, and summarize the key tools that are available. The humoral immune response to vaccination has been a particular focus of repertoire analyses, and we review the key conclusions and methods used in these studies.
新一代测序技术使以前所未有的详细程度研究生物体的抗体库成为可能，并且通过这样做来表征其在应对感染以及自身免疫和癌症等病理状况时的行为。抗体库的多态性带来了独特的挑战，这排除了许多常用的二代测序方法的使用，并且在考虑实验设计时需要进行权衡。我们概述了使用抗体库分析的主要背景，并总结了可用的关键工具。对疫苗接种的体液免疫反应一直是抗体库分析的一个特别重点，我们回顾了这些研究中使用的关键结论和方法。
