Boyd Scott D, Crowe James E
Department of Pathology, Stanford University, Stanford, CA 94305, United States.
Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232-0417, United States.
Curr Opin Immunol. 2016 Jun;40:103-9. doi: 10.1016/j.coi.2016.03.008. Epub 2016 Apr 8.
In the past decade, high-throughput DNA sequencing (HTS) methods and improved approaches for isolating antigen-specific B cells and their antibody genes have been applied in many areas of human immunology. This work has greatly increased our understanding of human antibody repertoires and the specific clones responsible for protective immunity or immune-mediated pathogenesis. Although the principles underlying selection of individual B cell clones in the intact immune system are still under investigation, the combination of more powerful genetic tracking of antibody lineage development and functional testing of the encoded proteins promises to transform therapeutic antibody discovery and optimization. Here, we highlight recent advances in this fast-moving field.
在过去十年中，高通量DNA测序（HTS）方法以及分离抗原特异性B细胞及其抗体基因的改进方法已应用于人类免疫学的许多领域。这项工作极大地增进了我们对人类抗体库以及负责保护性免疫或免疫介导发病机制的特定克隆的理解。尽管完整免疫系统中单个B细胞克隆的选择原则仍在研究中，但更强大的抗体谱系发育基因追踪与编码蛋白功能测试的结合有望改变治疗性抗体的发现与优化。在此，我们重点介绍这一快速发展领域的最新进展。
