Piek J J, Becker A E
Department of Pathology, University of Amsterdam, The Netherlands.
Am J Cardiovasc Pathol. 1989;2(4):301-8.
A technique is introduced to quantify the microvasculature of postmortem human hearts. The method combines coronary angiography with selective perfusion of the coronary arteries with differently labeled radioactive microspheres. The transmural distribution pattern of the microspheres (endocardium-epicardium ratios) reveals that rigor mortis is a factor that should be taken into account. In the majority of cases, however, noticeable effects of rigor mortis were absent. The results of the perfusion of 19 human hearts with 10 to 35 microns sized microspheres indicate that the distribution pattern of the microspheres is determined by arterioles with a minimal diameter of 35 microns. In a second series of 15 human hearts, a comparison in the pattern of distribution of microspheres was made between hearts with obstructive coronary artery disease (n = 9) and hearts without obstructive disease (n = 6). The results support the contention that the present technique allows us to quantify differences in collateral vascular supply. Hence, the method may be of use for the study of the microvascular bed in the zone at risk in hearts of patients who have died of myocardial infarction.
介绍了一种量化死后人类心脏微血管系统的技术。该方法将冠状动脉造影与用不同标记的放射性微球对冠状动脉进行选择性灌注相结合。微球的透壁分布模式（心内膜与心外膜比率）表明尸僵是一个应予以考虑的因素。然而，在大多数情况下，不存在明显的尸僵影响。用10至35微米大小的微球对19颗人类心脏进行灌注的结果表明，微球的分布模式由最小直径为35微米的小动脉决定。在第二组15颗人类心脏中，对患有阻塞性冠状动脉疾病的心脏（n = 9）和无阻塞性疾病的心脏（n = 6）的微球分布模式进行了比较。结果支持了这样的观点，即目前的技术使我们能够量化侧支血管供应的差异。因此，该方法可能有助于研究死于心肌梗死患者心脏中危险区域的微血管床。
