Bass Casie S, Redmer Dale A, Kaminski Samantha L, Grazul-Bilska Anna T
Department of Animal SciencesNorth Dakota State University, Fargo, North Dakota, USA.
Department of Animal SciencesNorth Dakota State University, Fargo, North Dakota, USA
Reproduction. 2017 Mar;153(3):253-265. doi: 10.1530/REP-16-0526. Epub 2016 Nov 29.
Functions of corpus luteum (CL) are influenced by numerous factors including hormones, growth and angiogenic factors, nutritional plane and dietary supplements such as arginine (Arg), a semi-essential amino acid and precursor for proteins, polyamines and nitric oxide (NO). The aim of this study was to determine if Arg supplementation to ewes fed different planes of nutrition influences: (1) progesterone (P4) concentrations in serum and luteal tissue, (2) luteal vascularity, cell proliferation, endothelial NO synthase (eNOS) and receptor (R) soluble guanylate cyclase β protein and mRNA expression and (3) luteal mRNA expression for selected angiogenic factors during the estrous cycle. Ewes (n = 111) were categorized by weight and randomly assigned to one of three nutritional planes: maintenance control (C), overfed (2× C) and underfed (0.6× C) beginning 60 days prior to onset of estrus. After estrus synchronization, ewes from each nutritional plane were assigned randomly to one of two treatments: Arg or saline. Serum and CL were collected at the early, mid and late luteal phases. The results demonstrated that: (1) nutritional plane affected ovulation rates, luteal vascularity, cell proliferation and NOS3, GUCY1B3, vascular endothelial growth factor (VEGF) and VEGFR2 mRNA expression, (2) Arg affected luteal vascularity, cell proliferation and NOS3, GUCY1B3, VEGF and VEGFR2 mRNA expression and (3) luteal vascularity, cell proliferation and the VEGF and NO systems depend on the stage of the estrous cycle. These data indicate that plane of nutrition and/or Arg supplementation can alter vascularization and expression of selected angiogenic factors in luteal tissue during the estrous cycle in sheep.
黄体（CL）的功能受多种因素影响，包括激素、生长和血管生成因子、营养水平以及饮食补充剂，如精氨酸（Arg），一种半必需氨基酸，是蛋白质、多胺和一氧化氮（NO）的前体。本研究的目的是确定向采食不同营养水平的母羊补充精氨酸是否会影响：（1）血清和黄体组织中的孕酮（P4）浓度；（2）黄体血管生成、细胞增殖、内皮型一氧化氮合酶（eNOS）以及受体（R）可溶性鸟苷酸环化酶β蛋白和mRNA表达；（3）发情周期中选定血管生成因子的黄体mRNA表达。母羊（n = 111）按体重分类，并在发情开始前60天随机分配到三种营养水平之一：维持对照（C）、过量饲喂（2×C）和不足饲喂（0.6×C）。发情同步后，每个营养水平的母羊随机分配到两种处理之一：精氨酸或生理盐水。在黄体早期、中期和晚期收集血清和黄体。结果表明：（1）营养水平影响排卵率、黄体血管生成、细胞增殖以及NOS3、GUCY1B3、血管内皮生长因子（VEGF）和VEGFR2 mRNA表达；（2）精氨酸影响黄体血管生成、细胞增殖以及NOS3、GUCY1B3、VEGF和VEGFR2 mRNA表达；（3）黄体血管生成、细胞增殖以及VEGF和NO系统取决于发情周期阶段。这些数据表明，营养水平和/或精氨酸补充可在绵羊发情周期中改变黄体组织中选定血管生成因子的血管化和表达。
