Grazul-Bilska Anna T, Thammasiri Jiratti, Kraisoon Aree, Reyaz Arshi, Bass Casie S, Kaminski Samantha L, Navanukraw Chainarong, Redmer Dale A
Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA.
Agricultural Biotechnology Research Center for Sustainable Economy (ABRCSE), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.
Theriogenology. 2018 Mar 1;108:7-15. doi: 10.1016/j.theriogenology.2017.11.008. Epub 2017 Nov 20.
To evaluate expression of progesterone receptor (PGR) AB in follicle stimulating hormone (FSH)-treated or non-treated sheep administered with arginine (Arg) or saline (Sal) fed a control (C), excess (O) or restricted (U) diet, uterine tissues were collected at the early, mid and/or late luteal phases. In exp. 1, ewes from each diet were randomly assigned to one of two treatments, Arg or Sal administration three times daily from day 0 of the first estrous cycle until uterine tissue collection. In exp. 2, ewes were injected twice daily with FSH on days 13-15 of the first estrous cycle. Uterine tissues were immunostained to detect PGR followed by image analysis. PGR were detected in luminal epithelium (LE), endometrial glands (EG), endometrial stroma (ES), myometrium (Myo), and endometrial and myometrial blood vessels. The percentage of PR-positive cells and/or intensity of staining were affected by phase of the estrous cycle, plane of nutrition, and/or FSH but not by Arg. In exp. 1, percentage of PGR-positive cells in LE and EG but not in ES and Myo was greater at the early and mid than late luteal phase, was not affected by plane of nutrition, and was similar in LE and EG. Intensity of staining was affected by phase of the estrous cycle and plane of nutrition in LE, EG and Myo, and was the greatest in LE, less in EG, and least in ES and Myo. In exp. 2, percentage of PGR-positive cells in LE, EG, ES and Myo was affected by phase of the estrous cycle, but not by plane of nutrition; was greater at the early than mid luteal phase; and was greatest in LE and EG, less in luminal (superficial) ES and Myo and least in deep ES. Intensity of staining was affected by phase of the estrous cycle and plane of nutrition in all compartments but ES, and was the greatest in LE and luminal EG, less in deep EG, and least in ES and Myo. Comparison of data for FSH (superovulated) and Sal-treated (non-superovulated) ewes demonstrated that FSH affected PR expression in all evaluated uterine compartments depending on plane of nutrition and phase of the estrous cycle. Thus, PGR are differentially distributed in uterine compartments, and PGR expression is affected by nutritional plane and FSH, but not Arg depending on phase of the estrous cycle. Such changes in dynamics of PGR expression indicate that diet plays a regulatory role and that FSH-treatment may alter uterine functions.
为了评估在接受促卵泡激素（FSH）处理或未处理、饲喂对照（C）、过量（O）或限饲（U）日粮并给予精氨酸（Arg）或生理盐水（Sal）的绵羊中孕激素受体（PGR）AB的表达情况，在黄体期早期、中期和/或晚期采集子宫组织。在实验1中，将每种日粮的母羊随机分为两种处理之一，从第一个发情周期的第0天开始至采集子宫组织，每天三次给予Arg或Sal。在实验2中，在第一个发情周期的第13 - 15天，母羊每天注射两次FSH。对子宫组织进行免疫染色以检测PGR，随后进行图像分析。在腔上皮（LE）、子宫内膜腺（EG）、子宫内膜基质（ES）、肌层（Myo）以及子宫内膜和肌层血管中检测到PGR。PR阳性细胞的百分比和/或染色强度受发情周期阶段、营养水平和/或FSH的影响，但不受Arg的影响。在实验1中，LE和EG中PGR阳性细胞的百分比在黄体期早期和中期高于晚期，ES和Myo中则不然，不受营养水平影响，且LE和EG中的相似。LE、EG和Myo中染色强度受发情周期阶段和营养水平影响，在LE中最大，EG中次之，ES和Myo中最小。在实验2中，LE、EG、ES和Myo中PGR阳性细胞的百分比受发情周期阶段影响，但不受营养水平影响；在黄体期早期高于中期；在LE和EG中最高，腔（浅层）ES和Myo中次之，深层ES中最低。除ES外，所有区域的染色强度均受发情周期阶段和营养水平影响，在LE和腔EG中最大，深层EG中次之，ES和Myo中最小。对FSH处理（超排）和Sal处理（未超排）母羊的数据比较表明，FSH根据营养水平和发情周期阶段影响所有评估子宫区域的PR表达。因此，PGR在子宫各区域的分布存在差异，PGR表达受营养水平和FSH影响，但不受Arg影响，具体取决于发情周期阶段。PGR表达动态的这种变化表明日粮起调节作用，且FSH处理可能改变子宫功能。
