N-乙酰葡糖胺介导的[具体内容1]和[具体内容2]在[具体对象]中的表达
N-acetylglucosamine-Mediated Expression of and in .
作者信息
Afzal Muhammad, Shafeeq Sulman, Manzoor Irfan, Henriques-Normark Birgitta, Kuipers Oscar P
机构信息
Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of GroningenGroningen, Netherlands; Department of Bioinformatics and Biotechnology, Government College UniversityFaisalabad, Pakistan.
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet Stockholm, Sweden.
出版信息
Front Cell Infect Microbiol. 2016 Nov 16;6:158. doi: 10.3389/fcimb.2016.00158. eCollection 2016.
In this study, we have explored the transcriptomic response of D39 to N-acetylglucosamine (NAG). Transcriptome comparison of D39 wild-type grown in chemically defined medium (CDM) in the presence of 0.5% NAG to that grown in the presence of 0.5% glucose revealed elevated expression of many genes/operons, including , and . We have further confirmed the NAG-dependent expression of , and by β-galactosidase assays. and are putatively regulated by a transcriptional regulator NagR. We predicted the operator site of NagR ( site) in P, P, and P, which was further confirmed by mutating the predicted site in the respective promoters (, and ). Growth comparison of Δ, Δ, and Δ with the D39 wild-type demonstrates that and are essential for D39 to grow in the presence of NAG as a sole carbon source. Furthermore, deletion of shows that CcpA has no effect on the expression of , and in the presence of NAG in . .
在本研究中，我们探究了D39对N - 乙酰葡糖胺（NAG）的转录组反应。将在含有0.5% NAG的化学限定培养基（CDM）中生长的D39野生型与在含有0.5%葡萄糖的培养基中生长的D39野生型进行转录组比较，结果显示许多基因/操纵子的表达上调，包括 ，以及 。我们通过β - 半乳糖苷酶测定进一步证实了 、 和 的NAG依赖性表达。 和 可能受转录调节因子NagR调控。我们预测了P、P和P中NagR的操纵子位点（ 位点），通过在各自启动子（ 、 和 ）中突变预测的 位点进一步证实了这一点。Δ、Δ和Δ与D39野生型的生长比较表明， 和 对于D39在以NAG作为唯一碳源的情况下生长至关重要。此外， 的缺失表明在 中存在NAG的情况下，CcpA对 、 和 的表达没有影响。
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