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烟酸介导的基因表达以及NiaR作为[具体基因名称]在[具体物种名称]中对[相关基因名称]的转录抑制作用及角色。 需注意，原文中部分基因名称缺失，以上是根据格式补齐后的完整译文表述，实际翻译时需根据准确的原文基因和物种信息进行准确翻译。

Niacin-mediated Gene Expression and Role of NiaR as a Transcriptional Repressor of , and in .

作者信息

Afzal Muhammad, Kuipers Oscar P, Shafeeq Sulman

机构信息

Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of GroningenGroningen, Netherlands; Department of Bioinformatics and Biotechnology, Government College UniversityFaisalabad, Pakistan.

Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Groningen, Netherlands.

出版信息

Front Cell Infect Microbiol. 2017 Mar 9;7:70. doi: 10.3389/fcimb.2017.00070. eCollection 2017.

DOI:10.3389/fcimb.2017.00070
PMID:28337428
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5343564/
Abstract

NAD (Nicotinamide Adenine Dinucleotide) biosynthesis is vital for bacterial physiology and plays an important role in cellular metabolism. A naturally occurring vitamin B complex, niacin (nicotinic acid), is a precursor of coenzymes NAD and NADP. Here, we study the impact of niacin on global gene expression of D39 and elucidate the role of NiaR as a transcriptional regulator of , and . Transcriptome comparison of the D39 wild-type grown in chemically defined medium (CDM) with 0 to 10 mM niacin revealed elevated expression of various genes, including , and that are putatively involved in the transport and utilization of niacin. Niacin-dependent expression of these genes is confirmed by promoter fusion studies. Moreover, the role of transcriptional regulator NiaR in the regulation of these genes is explored by DNA microarray analysis. Our transcriptomic comparison of D39 Δ to D39 wild-type revealed that the transcriptional regulator NiaR acts as a transcriptional repressor of , and . NiaR-dependent regulation of , and is further confirmed by promoter fusion studies. The putative operator site of NiaR (5'-TACWRGTGTMTWKACASYTRWAW-3') in the promoter regions of , and is predicted and further confirmed by promoter mutational experiments.

摘要

烟酰胺腺嘌呤二核苷酸（NAD）的生物合成对细菌生理至关重要，在细胞代谢中发挥重要作用。天然存在的复合维生素B，烟酸（尼克酸），是辅酶NAD和NADP的前体。在此，我们研究了烟酸对D39全局基因表达的影响，并阐明了NiaR作为[具体基因1]和[具体基因2]转录调节因子的作用。在含有0至10 mM烟酸的化学限定培养基（CDM）中培养的D39野生型的转录组比较显示，包括[具体基因3]、[具体基因4]和[具体基因5]在内的各种基因表达升高，这些基因可能参与烟酸的转运和利用。这些基因的烟酸依赖性表达通过启动子融合研究得到证实。此外，通过DNA微阵列分析探索了转录调节因子NiaR在这些基因调控中的作用。我们对D39 Δ[具体基因6]与D39野生型的转录组比较显示，转录调节因子NiaR作为[具体基因1]、[具体基因2]和[具体基因3]的转录抑制因子发挥作用。启动子融合研究进一步证实了NiaR对[具体基因1]、[具体基因2]和[具体基因3]的依赖性调控。预测了NiaR在[具体基因1]、[具体基因2]和[具体基因3]启动子区域的假定操纵位点（5'-TACWRGTGTMTWKACASYTRWAW-3'），并通过启动子突变实验进一步证实。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d13/5343564/6b3d99929821/fcimb-07-00070-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d13/5343564/a3fc4ca82158/fcimb-07-00070-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d13/5343564/5ecc71c861f2/fcimb-07-00070-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d13/5343564/1e8e2c13ab9e/fcimb-07-00070-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d13/5343564/1b8c8841f3dd/fcimb-07-00070-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d13/5343564/6b3d99929821/fcimb-07-00070-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d13/5343564/a3fc4ca82158/fcimb-07-00070-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d13/5343564/5ecc71c861f2/fcimb-07-00070-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d13/5343564/1e8e2c13ab9e/fcimb-07-00070-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d13/5343564/1b8c8841f3dd/fcimb-07-00070-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d13/5343564/6b3d99929821/fcimb-07-00070-g0005.jpg

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