Hlavaty Juraj, Wolfesberger Birgitt, Hauck Marlene, Obermayer-Pietsch Barbara, Fuchs-Baumgartinger Andrea, Miller Ingrid, Walter Ingrid
Institute of Anatomy, Histology and Embryology, University of Veterinary Medicine, Vienna, Austria.
Clinic for Companion Animal Medicine, Unit for Internal Medicine, University of Veterinary Medicine, Vienna, Austria.
Histol Histopathol. 2017 Aug;32(8):805-816. doi: 10.14670/HH-11-848. Epub 2016 Nov 30.
Biological features of canine osteosarcomas (OS) differ markedly from those found in feline and resemble more human osteosarcomas, in particular for their high rate of metastasis and poor prognosis. Ezrin, radixin and moesin are members of the ERM protein family and link the actin cytoskeleton with the cell membrane. Ezrin and moesin have been shown to be of prognostic significance in tumor progression due to their role in the metastatic process. The objective of this study was to analyze ezrin and moesin protein expression in a series of dog (n = 16) and cat (n = 8) osteosarcoma samples using immunohistochemistry and western blot techniques. We found that cat OS have a higher moesin expression compared to dog OS, however, the active phosphorylated forms of moesin and ezrin Tyr353 were more abundant in the dog samples. A statistically significant difference was found for the low and high immunohistochemical scores of ezrin and pan-phospho-ERM proteins between cat and dog. Although phospho-ezrin Thr567 was higher in feline OS, the membranous localization in dog OS samples indicates the presence of the biologically active form. Therefore, the observed differences in phosphorylated forms of ezrin and moesin status should be further studied to demonstrate if they are relevant for different biological behavior between dog and cat OS.
犬骨肉瘤（OS）的生物学特征与猫骨肉瘤明显不同，更类似于人类骨肉瘤，尤其是在转移率高和预后差方面。埃兹蛋白（ezrin）、根蛋白（radixin）和膜突蛋白（moesin）是ERM蛋白家族的成员，它们将肌动蛋白细胞骨架与细胞膜连接起来。由于埃兹蛋白和膜突蛋白在转移过程中的作用，它们已被证明在肿瘤进展中具有预后意义。本研究的目的是使用免疫组织化学和蛋白质印迹技术分析一系列犬（n = 16）和猫（n = 8）骨肉瘤样本中埃兹蛋白和膜突蛋白的表达。我们发现，与犬骨肉瘤相比，猫骨肉瘤的膜突蛋白表达更高，然而，膜突蛋白和埃兹蛋白Tyr353的活性磷酸化形式在犬样本中更为丰富。在猫和犬之间，埃兹蛋白和泛磷酸化ERM蛋白的低和高免疫组织化学评分存在统计学上的显著差异。尽管磷酸化埃兹蛋白Thr567在猫骨肉瘤中更高，但在犬骨肉瘤样本中的膜定位表明存在生物活性形式。因此，应进一步研究观察到的埃兹蛋白和膜突蛋白磷酸化形式状态的差异，以证明它们是否与犬和猫骨肉瘤之间不同的生物学行为相关。
