Emami Nima C, Leong Lancelote, Wan Eunice, Van Blarigan Erin L, Cooperberg Matthew R, Tenggara Imelda, Carroll Peter R, Chan June M, Witte John S, Simko Jeffry P
Program in Biological and Medical Informatics, University of California, San Francisco, California.
Department of Epidemiology and Biostatistics, University of California, San Francisco, California.
Prostate. 2017 Mar;77(4):425-434. doi: 10.1002/pros.23283. Epub 2016 Nov 30.
Benign tissue from a tumor-containing organ is commonly the only available source for obtaining a patient's unmutated genome for use in cancer research. While it is critical to identify histologically normal tissue that is independent of the tumor lineage, few additional considerations are applied to the choice of this material for such measurements.
Normal formalin-fixed, paraffin-embedded seminal vesicle, and urethral tissues, in addition to whole blood, were collected from 31 prostate cancer patients having undergone radical prostatectomy. Genotype concordance was evaluated for DNA from each tissue source in relation to whole blood.
Overall, there was a greater genotype call rate for DNA derived from urethral tissue (97.0%) in comparison with patient-matched seminal vesicle tissues (95.9%, P = 0.0015). Furthermore, with reference to patient-matched whole blood, urethral samples exhibited higher genotype concordance (94.1%) than that of seminal vesicle samples (92.5%, P = 0.035).
These findings highlight the heterogeneity between diverse sources of DNA in genotype measurement and motivate the consideration of normal tissue biases in tumor-normal analyses. Prostate 77: 425-434, 2017. © 2016 Wiley Periodicals, Inc.
来自含有肿瘤器官的良性组织通常是获取患者未突变基因组用于癌症研究的唯一可用来源。虽然识别独立于肿瘤谱系的组织学正常组织至关重要，但在选择用于此类测量的材料时，几乎没有其他需要考虑的因素。
从31例接受根治性前列腺切除术的前列腺癌患者中收集正常福尔马林固定、石蜡包埋的精囊和尿道组织以及全血。评估每个组织来源的DNA与全血的基因型一致性。
总体而言，与患者匹配的精囊组织（95.9%，P = 0.0015）相比，尿道组织来源的DNA基因型检出率更高（97.0%）。此外，与患者匹配的全血相比，尿道样本的基因型一致性（94.1%）高于精囊样本（92.5%，P = 0.035）。
这些发现突出了基因型测量中不同DNA来源之间的异质性，并促使在肿瘤-正常分析中考虑正常组织偏差。《前列腺》77: 425 - 434, 2017。© 2016威利期刊公司。
