Yanai J, Guttman R, Stern E
Melvin A. and Eleanor Ross Laboratory for Studies in Neural Birth Defects, Department of Anatomy and Embryology, Hebrew University-Hadassah Medical School, Israel.
Biol Neonate. 1989;56(2):109-16. doi: 10.1159/000243110.
Results of this investigation show a differential effect of early barbiturate administration on behaviors of mice of two inbred strains in the open field and in the barrier cage. Pups of the inbred strains DBA/l and C57BL/10 were fostered by HS/Ibg dams and were given a daily injection of 50 mg/kg sodium phenobarbital on postnatal days 2-21 (treated groups); control pups received vehicle injections. At 28 and 30 days the animals were tested in an open field and in a barrier cage. The DBA/l mice responded differently from C57BL/10 to early barbiturate treatment. Treated DBA/l mice showed a marked reduction from control levels in open-field locomotion and grooming but not in defecation. In the barrier test, there were reductions in jumping, peeking and grooming and increase in defecation. The differences between controls and treated in the C57BL/10 strain were small except for barrier jumping and peeking. It is suggested that genotype (strain) - environment (drug) interaction exists for the effect of early phenobarbital administration on behavior: DBA/l was sharply affected by the drug, while the behavior of C57BL/10 was little altered by neonatal phenobarbital administration.
本次研究结果显示，早期给予巴比妥酸盐对两个近交系小鼠在旷场和屏障笼中的行为有不同影响。近交系DBA/l和C57BL/10的幼崽由HS/Ibg母鼠抚养，并在出生后第2至21天每天注射50mg/kg苯巴比妥钠（处理组）；对照幼崽注射赋形剂。在28天和30天时，对动物进行旷场和屏障笼测试。早期巴比妥酸盐处理后，DBA/l小鼠与C57BL/10小鼠的反应不同。经处理的DBA/l小鼠在旷场中的运动和梳理行为较对照组明显减少，但排便行为无明显变化。在屏障测试中，跳跃、窥视和梳理行为减少，排便行为增加。C57BL/10品系对照组和处理组之间的差异较小，除了屏障跳跃和窥视行为。这表明早期给予苯巴比妥对行为的影响存在基因型（品系）-环境（药物）相互作用：DBA/l对药物反应强烈，而新生期给予苯巴比妥对C57BL/10的行为影响较小。
