de Mendonca M, Appel M, Bidault J, Masson-Pierre D, Terrier E
Vox Sang. 1978 Sep;35(3):184-92. doi: 10.1111/j.1423-0410.1978.tb02920.x.
A novel procedure for storing blood at a controlled pH consists in collecting blood in a pH 8.20, Tris-CPD solution and storing it in a special recipient including a gas permeable membrane under a CO2 atmosphere. The recipient is placed in an atmosphere of variable CO2 content, so that the initial alkalinity of the preservative is balanced by dissolved CO2, the proportion of which is diminished when lactate production increases with storage. 2,3-DPG and ATP were studied at three different pH levels of approximately 7.25, 7.45, and 7.65 at 4 degrees C. The best pH for the simultaneous maintenance of 2,3-DPG and ATP was 7.65. Under these conditions, 2,3-DPG is maintained at its initial level and ATP at 55% of its initial level at the 30th day. Lactate production is linear and hemolysis moderate.
一种在可控pH值下储存血液的新方法包括：将血液收集到pH值为8.20的Tris - CPD溶液中，并将其储存在一个特殊的容器中，该容器包括一个在CO₂气氛下的透气膜。将该容器置于CO₂含量可变的气氛中，以便防腐剂的初始碱度由溶解的CO₂平衡，当储存时乳酸产量增加时，溶解的CO₂比例会降低。在4℃下，研究了在大约7.25、7.45和7.65这三个不同pH水平下的2,3 - DPG和ATP。同时维持2,3 - DPG和ATP的最佳pH值为7.65。在这些条件下，在第30天时，2,3 - DPG维持在其初始水平，而ATP维持在其初始水平的55%。乳酸产量呈线性，溶血程度适中。
