闪光光解后MbCO晶体中的电荷运动。
Charge motion in MbCO crystals after flash photolysis.
作者信息
Iben I E, Keszthelyi L, Ringe D, Váró G
机构信息
Department of Physics, University of Illinois, Urbana 61801.
出版信息
Biophys J. 1989 Sep;56(3):459-63. doi: 10.1016/S0006-3495(89)82693-1.
Abstract
Charge motion accompanying the dissociation and recombination of carbon monoxide to oriented myoglobin crystals has been observed. The magnitude of the electrical signals detected after photodissociation by electrodes on either side of MbCO crystals of type A is consistent with the x-ray data showing that the doubly charged iron ion lies in the mean heme plane when a ligand is bound and moves out of the plane when deligated. Beyond 10 ms, the time development of the electrical signal is consistent with the kinetics observed optically after flash photolysis on the same crystals.
摘要
已观察到一氧化碳与定向肌红蛋白晶体解离和重组时伴随的电荷运动。A 型 MbCO 晶体两侧电极在光解离后检测到的电信号大小与 x 射线数据一致，该数据表明当配体结合时，双电荷铁离子位于平均血红素平面内，而去配体时则移出该平面。在 10 毫秒之后，电信号的时间发展与在相同晶体上进行闪光光解后光学观察到的动力学一致。
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