Peixoto Renata Stavracakis, Hacker Elena, Antunes Camila Azevedo, Weerasekera Dulanthi, Dias A A, Martins Carlos Alberto, Hirata Raphael, Santos Kátia Regina Netto Dos, Burkovski Andreas, Mattos-Guaraldi Ana Luíza
Professur für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Department of Medical Microbiology, Institute of Microbiology, Rio de Janeiro Federal University (IMPPG/UFRJ), Rio de Janeiro, RJ, Brazil.
J Med Microbiol. 2016 Nov;65(11):1311-1321. doi: 10.1099/jmm.0.000362.
Corynebacterium diphtheriae is typically recognized as a colonizer of the upper respiratory tract (respiratory diphtheria) and the skin (cutaneous diphtheria). However, different strains of Corynebacteriumdiphtheriae can also cause invasive infections. In this study, the characterization of a non-toxigenic Corynebacteriumdiphtheriae strain (designated BR-INCA5015) isolated from osteomyelitis in the frontal bone of a patient with adenoid cystic carcinoma was performed. Pathogenic properties of the strain BR-INCA5015 were tested in a Caenorhabditis elegans survival assay showing strong colonization and killing by this strain. Survival rates of 3.8±2.7 %, 33.6±7.3 % and 0 % were observed for strains ATCC 27010T, ATCC 27012 and BR-INCA5015, respectively, at day 7. BR-INCA5015 was able to colonize epithelial cells, showing elevated capacity to adhere to and survive within HeLa cells compared to other Corynebacteriumdiphtheriae isolates. Intracellular survival in macrophages (THP-1 and RAW 264.7) was significantly higher compared to control strains ATCC 27010T (non-toxigenic) and ATCC 27012 (toxigenic). Furthermore, the ability of BR-INCA5015 to induce osteomyelitis was confirmed by in vivo assay using Swiss Webster mice.
白喉棒状杆菌通常被认为是上呼吸道（呼吸道白喉）和皮肤（皮肤白喉）的定植菌。然而，不同菌株的白喉棒状杆菌也可引起侵袭性感染。在本研究中，对从一名腺样囊性癌患者额骨骨髓炎中分离出的非产毒白喉棒状杆菌菌株（命名为BR-INCA5015）进行了特性分析。在秀丽隐杆线虫生存试验中测试了BR-INCA5015菌株的致病特性，结果显示该菌株具有很强的定植和杀伤能力。在第7天，ATCC 27010T菌株、ATCC 27012菌株和BR-INCA5015菌株的生存率分别为3.8±2.7%、33.6±7.3%和0%。BR-INCA5015能够定植于上皮细胞，与其他白喉棒状杆菌分离株相比，其黏附并在HeLa细胞内存活的能力有所提高。与对照菌株ATCC 27010T（非产毒）和ATCC 27012（产毒）相比，BR-INCA5015在巨噬细胞（THP-1和RAW 264.7）中的细胞内存活率显著更高。此外，使用瑞士韦伯斯特小鼠进行的体内试验证实了BR-INCA5015诱导骨髓炎的能力。
