Department of Clinical Laboratory, Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang, Zhejiang, China.
Department of Biomedical Sciences Laboratory, Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang, Zhejiang, China.
BMC Genom Data. 2021 Oct 16;22(1):42. doi: 10.1186/s12863-021-00998-9.
Sporadic cases of infection with non-toxigenic Corynebacterium diphtheriae (C. diphtheriae) isolates have been reported in regions covered by the Diphtheria-Tetanus-Pertussis vaccine, but no information describing the whole genome of non-toxigenic strains collected in China is available. Therefore, in this work, the complete genome of a non-toxigenic strain of C. diphtheriae from a hospital located in southeastern China was performed.
This non-toxigenic isolate belonged to the belfanti biotype and possessed a unique ST (assigned as ST799 in pubMLST). ErmX was present in the genome sequence and this isolate owned the resistance to erythromycin and clindamycin. Genes coding for virulence factors involved in adherence, iron-uptake and regulation of diphtheria toxin were also found. Two genes were involved in the interaction between pathogen and host. The phylogenetic analysis revealed that this newly isolated strain was similar to the strain NCTC10838, CMCNS703 and CHUV2995.
Non-toxigenic C. diphtheriae strain contained virulence factors, thus it is able to cause an infectious disease, aspect that could be clarified by performing the whole genome sequencing analysis.
在白喉-破伤风-百日咳疫苗覆盖的地区，已经报道了散发性非产毒白喉棒状杆菌（C. diphtheriae）分离株感染病例，但尚无关于在中国采集的非产毒菌株全基因组的信息。因此，在这项工作中，对来自中国东南部一家医院的非产毒 C. diphtheriae 进行了全基因组测序。
该非产毒分离株属于 Belfanti 生物型，具有独特的 ST（在 pubMLST 中被指定为 ST799）。基因组序列中存在 ErmX，该分离株对红霉素和克林霉素具有耐药性。编码与粘附、铁摄取和白喉毒素调节相关的毒力因子的基因也被发现。有两个基因参与了病原体与宿主之间的相互作用。系统发育分析表明，新分离的菌株与 NCTC10838 株、CMCNS703 株和 CHUV2995 株相似。
非产毒白喉棒状杆菌菌株含有毒力因子，因此能够引起传染病，通过全基因组测序分析可以澄清这一点。
