Carr P A, Yamamoto T, Karmy G, Baimbridge K G, Nagy J I
Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Brain Res. 1989 Sep 11;497(1):163-70. doi: 10.1016/0006-8993(89)90983-9.
Sections of lumbar dorsal root ganglia from rat were analyzed by immunohistochemical techniques to determine the size distribution and numbers of cells containing parvalbumin and calbindin D28k and to establish their coexistence relationships with each other and with cells containing calcitonin gene-related peptide (CGRP). The proportion of ganglia cells containing parvalbumin and calbindin D28k was 14% and 22%, respectively. The majority of cells immunoreactive for these proteins were of the large A type. Parvalbumin was colocalized almost completely (greater than 99%) with with calbindin D28k and minimally (less than 1%) with CGRP. Only 9% of the calbindin D28k-positive cells were immunoreactive for CGRP.
采用免疫组织化学技术分析大鼠腰段背根神经节切片，以确定含小白蛋白和钙结合蛋白D28k的细胞的大小分布和数量，并确定它们彼此之间以及与含降钙素基因相关肽（CGRP）的细胞的共存关系。含小白蛋白和钙结合蛋白D28k的神经节细胞比例分别为14%和22%。这些蛋白免疫反应阳性的大多数细胞为大A型。小白蛋白几乎完全（大于99%）与钙结合蛋白D28k共定位，与CGRP的共定位极少（小于1%）。仅9%的钙结合蛋白D28k阳性细胞对CGRP呈免疫反应。
