Huang Bisheng, Zhai Wei, Hu Guanghui, Huang Chi, Xie Tiancheng, Zhang Jingwei, Xu Yunfei
Department of Urology, Shanghai Tenth People's Hospital, Tongji University 301 Yanchang Road, Shanghai 200072, China.
Department of Urology, Shanghai Tenth People's Hospital, Tongji University301 Yanchang Road, Shanghai 200072, China; Department of First Clinical Medical College, Nanjing Medical UniversityNanjing, Jiangsu, China.
Am J Transl Res. 2016 Nov 15;8(11):4705-4715. eCollection 2016.
Accumulating evidence suggested that microRNA (miRNA) plays important regulatory roles in the initiation and development of various cancers. Previous study showed that microRNA-206 (miR-206) is dysregulated in human bladder cancer tissues, however, the biological function and underlying mechanisms of miR-206 in human bladder cancer remain unknown. In the present study, we aimed to investigate the clinical significance of miR-206 and its target gene YRDC in human bladder cancer, and to determine its effects on oncogenic phenotypes of this disease. Our results showed that miR-206 expression was downregulated significantly in bladder cancer tissues and cell lines compared with adjacent normal bladder tissues and human bladder epithelial immortalized SV-HUC-1 cell line, respectively. Overexpression of miR-206 reduced the expression of YRDC and inhibited bladder cancer cell proliferation, colony formation, migration, invasion and induced cell cycle arrest at G0/G1 phase. In addition, knockdown of YRDC exhibited similar effects with miR-206 overexpression in bladder cancer cells and restoration of YRDC partially reversed the effects of miR-206 in bladder cancer cells. These findings indicated that mir-206 might be a novel target for bladder cancer therapy by targeting YRDC.
越来越多的证据表明，微小RNA（miRNA）在各种癌症的发生和发展中发挥着重要的调节作用。先前的研究表明，微小RNA-206（miR-206）在人膀胱癌组织中表达失调，然而，miR-206在人膀胱癌中的生物学功能及潜在机制仍不清楚。在本研究中，我们旨在探讨miR-206及其靶基因YRDC在人膀胱癌中的临床意义，并确定其对该疾病致癌表型的影响。我们的结果显示，与相邻正常膀胱组织和人膀胱上皮永生化SV-HUC-1细胞系相比，miR-206在膀胱癌组织和细胞系中的表达分别显著下调。miR-206的过表达降低了YRDC的表达，并抑制了膀胱癌细胞的增殖、集落形成、迁移、侵袭，并诱导细胞周期停滞在G0/G1期。此外，敲低YRDC在膀胱癌细胞中表现出与miR-206过表达相似的作用，而恢复YRDC的表达部分逆转了miR-206在膀胱癌细胞中的作用。这些发现表明，miR-206可能通过靶向YRDC成为膀胱癌治疗的一个新靶点。
