Kiba M, Yamamura M, Kogata M, Hamada Y, Yamamoto M
Department of Surgery Kansai Medical University Osaka, Japan.
Cancer Invest. 1989;7(3):237-42. doi: 10.3109/07357908909039843.
Soluble tumor-associated antigens were isolated from Lewis lung carcinoma (3LL) cells by detergent solubilization and purified by affinity chromatography on peanut agglutinin (PNA)-agarose. Subcutaneous injection of PNA-binding glyco-related antigen into 3LL-bearing male C57BL/6 mice significantly augmented delayed-type hypersensitivity (DTH), and also inhibited the growth of the primary 3LL tumors. The effects of combined immunotherapy and chemotherapy with this antigen and cyclophosphamide (CY) were examined following excision of 3LL by determining the number of pulmonary surface nodules and wet weight of the lungs. Injection of PNA-binding glyco-related antigen and CY significantly inhibited pulmonary metastasis and prolonged the survival of the mice. The spleen cells obtained from mice with combined treatment showed higher neutralizing activity against 3LL cells than other groups. Therefore, combined immuno and chemotherapy may be useful in preventing metastasis.
通过去污剂溶解从Lewis肺癌（3LL）细胞中分离出可溶性肿瘤相关抗原，并通过在花生凝集素（PNA）-琼脂糖上进行亲和层析进行纯化。将与PNA结合的糖相关抗原皮下注射到携带3LL的雄性C57BL/6小鼠中，可显著增强迟发型超敏反应（DTH），并抑制原发性3LL肿瘤的生长。在切除3LL后，通过测定肺表面结节数量和肺湿重，研究了该抗原与环磷酰胺（CY）联合免疫治疗和化疗的效果。注射与PNA结合的糖相关抗原和CY可显著抑制肺转移并延长小鼠存活时间。联合治疗小鼠的脾细胞对3LL细胞的中和活性高于其他组。因此，联合免疫和化疗可能有助于预防转移。
