Adachi M, Mizuta T, Yokota M, Shibusawa H, Yamamura M, Hamada Y, Uetsuji S, Yamamoto M, Miwa H
Anticancer Res. 1984 Jan-Apr;4(1-2):1-3.
Receptors for peanut agglutinin (PNA) were isolated from Lewis lung carcinoma cells (3LL) by detergent solubilization and affinity chromatography on PNA-agarose. The isolated receptors showed heterogeneous yet distinct molecular species when they were analyzed by SDS gel electrophoresis. In vivo inoculation of the isolated receptors could induce potent cytotoxic effector cells against 3LL cells, which were detected by Winn's tumor neutralization assay. The PNA receptors were also effective in preventing the settlement of the intravenously injected 3LL tumors in the lung. These findings suggest that PNA receptors can be used for immunological therapy of certain cancers.
通过去污剂增溶和在花生凝集素（PNA）-琼脂糖上进行亲和层析，从Lewis肺癌细胞（3LL）中分离出PNA受体。当通过SDS凝胶电泳分析时，分离出的受体呈现出异质性但又不同的分子种类。对分离出的受体进行体内接种可诱导产生针对3LL细胞的强效细胞毒性效应细胞，这通过Winn肿瘤中和试验得以检测。PNA受体在预防静脉注射的3LL肿瘤在肺部的定植方面也有效。这些发现表明PNA受体可用于某些癌症的免疫治疗。
