Muss H B, Kute T E, Case L D, Smith L R, Booher C, Long R, Kammire L, Gregory B, Brockschmidt J K
Cancer Center of Wake Forest University, Winston-Salem, North Carolina.
Cancer. 1989 Nov 1;64(9):1894-900. doi: 10.1002/1097-0142(19891101)64:9<1894::aid-cncr2820640923>3.0.co;2-k.
Flow cytometry (FC) analysis including DNA index (ploidy status) and cell kinetics (%S and %S + G2/M) was done on frozen tissue of the primary lesions of 101 women with node negative (N-) breast cancer who were studied prospectively. Currently, 19% (19/101) of the patients have recurred. No significant relations have been found between recurrence or survival and age, estrogen/progesterone receptor status, tumor size, and tumor type. The DNA index (ploidy) was not related to any clinical variable, time to recurrence, or survival. Aneuploid tumors did, however, have significantly higher %S phase activity. Patients with %S activity less than or equal to the median value were significantly different from those patients with %S above the median. They were older and had a higher frequency of ER/PR positive and well- or moderately differentiated tumors. Patients with %S + G2/M greater than the median value showed shorter time to recurrence (P = .055) and shorter survival (P = .006), whereas %S alone was significantly associated only with survival. Multivariate analysis showed that neither DNA index nor cell kinetics was significantly associated with time to relapse. DNA index was not significantly associated with survival; %S was of borderline significance whereas %S + %G2/M was a significant independent predictor of survival. Although FC data may provide independent information related to survival in N-women, additional research in a larger number of patients is needed to define its precise role in patient management.
对101例接受前瞻性研究的淋巴结阴性（N-）乳腺癌女性原发灶的冷冻组织进行了流式细胞术（FC）分析，包括DNA指数（倍体状态）和细胞动力学（%S期和%S + G2/M期）。目前，19%（19/101）的患者出现了复发。在复发或生存与年龄、雌激素/孕激素受体状态、肿瘤大小和肿瘤类型之间未发现显著相关性。DNA指数（倍体）与任何临床变量、复发时间或生存均无关联。然而，非整倍体肿瘤的%S期活性显著更高。%S活性小于或等于中位数的患者与%S高于中位数的患者有显著差异。他们年龄更大，ER/PR阳性以及高分化或中分化肿瘤的发生率更高。%S + G2/M大于中位数的患者复发时间较短（P = .055），生存时间较短（P = .006），而单独的%S仅与生存显著相关。多因素分析表明，DNA指数和细胞动力学均与复发时间无显著关联。DNA指数与生存无显著关联；%S具有临界显著性，而%S + %G2/M是生存的显著独立预测因子。尽管FC数据可能提供与N-女性生存相关的独立信息，但需要对更多患者进行进一步研究以确定其在患者管理中的精确作用。
