Galilea San Blas Oscar, Moreno Sanz Fernando, Herrero Espílez Pilar, Sainz Menéndez Rosa María, Mayo Barallo Juan Carlos, Marchante-Gayón Juan Manuel, García Alonso José Ignacio
Department of Physical and Analytical Chemistry, University of Oviedo, Julian Clavería 8, 33006 Oviedo, Spain.
Department of Biochemistry and Molecular Biology, University of Oviedo, Spain.
J Trace Elem Med Biol. 2017 Jan;39:155-161. doi: 10.1016/j.jtemb.2016.09.006. Epub 2016 Sep 24.
Sulfur isotopic enrichment of urine metabolites in healthy and prostate cancer mice using S enriched yeast and High Performance Liquid Chromatography coupled to Multicollector Inductively Coupled Plasma Mass Spectrometry (HPLC-MC-ICP-MS) has been evaluated. A 30 weeks experiment (since the eleventh to the fortieth week of life) was carried out collecting the urine of three healthy mice and three transgenic mice with prostate cancer during 24h after a single oral administration of a S enriched yeast slurry. The isotopic enrichment of different sulphur metabolites was monitored by coupling a C18 reverse phase HPLC column with a multicollector ICP-MS using a membrane desolvating system. Quantification of sulfur in the chromatographic peaks was carried out by post-column isotope dilution using a S enriched spike. Differences between the S enrichment in the urine metabolites of healthy and prostate cancer mice were found from the beginning of the disease. Both populations could be differentiated using a principal component analysis (PCA). Finally, 7 unknown mice were correctly classified in each population using a linear discriminant analysis.
利用富含硫的酵母以及高效液相色谱-多接收电感耦合等离子体质谱联用技术（HPLC-MC-ICP-MS），对健康小鼠和前列腺癌小鼠尿液代谢物中的硫同位素富集情况进行了评估。开展了一项为期30周的实验（从出生后第11周持续至第40周），在单次口服富含硫的酵母悬液后24小时内，收集三只健康小鼠和三只前列腺癌转基因小鼠的尿液。通过将C18反相高效液相色谱柱与采用膜去溶剂化系统的多接收电感耦合等离子体质谱联用，监测不同硫代谢物的同位素富集情况。使用富含硫的内标物通过柱后同位素稀释法对色谱峰中的硫进行定量。从疾病初期就发现了健康小鼠和前列腺癌小鼠尿液代谢物中硫富集情况的差异。使用主成分分析（PCA）可以区分这两种小鼠群体。最后，通过线性判别分析，在每个群体中7只未知小鼠都被正确分类。
