Jensen Berit Packert, Smith Christopher, Wilson Ian D, Weidolf Lars
Department of Drug Metabolism and Pharmacokinetics, AstraZeneca, Mereside, Alderley Park, Macclesfield SK10 4TG, UK.
Rapid Commun Mass Spectrom. 2004;18(2):181-3. doi: 10.1002/rcm.1312.
The use of high-performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICPMS) with sulphur-specific detection was investigated as a method for obtaining metabolite profiles for the drug omeprazole administered as a 1:1 mixture of (32)S- and (34)S-labelled material. Analysis based on the monitoring of the chromatographic eluent at either m/z 32 or 34 was not successful due to insufficient sensitivity caused by interferences from polyatomic ions. However, reaction of sulphur with oxygen in the hexapole collision cell, combined with monitoring at m/z 48 (for (32)S) or m/z 50 (for (34)S), provided a facile method for metabolite profiling. Detection of m/z 48 was superior in sensitivity to detection of m/z 50.
研究了使用具有硫特异性检测功能的高效液相色谱/电感耦合等离子体质谱法（HPLC/ICPMS），作为获取以（32）S和（34）S标记物质1:1混合物形式给药的药物奥美拉唑代谢物谱的一种方法。基于在m/z 32或34处监测色谱洗脱液的分析未成功，原因是多原子离子的干扰导致灵敏度不足。然而，硫在六极碰撞池中与氧的反应，结合在m/z 48（对于（32）S）或m/z 50（对于（34）S）处的监测，提供了一种简便的代谢物谱分析方法。检测m/z 48的灵敏度优于检测m/z 50。
