Serra-Compte Albert, Álvarez-Muñoz Diana, Rodríguez-Mozaz Sara, Barceló Damià
ICRA-Catalan Institute for Water Research, H(2)O Building, Scientific and Technological Park of the University of Girona, Emili Grahit 101, 17003 Girona, Spain.
ICRA-Catalan Institute for Water Research, H(2)O Building, Scientific and Technological Park of the University of Girona, Emili Grahit 101, 17003 Girona, Spain.
Food Chem Toxicol. 2017 Jun;104:3-13. doi: 10.1016/j.fct.2016.11.031. Epub 2016 Nov 28.
The presence of antibiotics in seafood for human consumption may pose a risk for consumers. A methodology for the analysis of antibiotics in seafood based on QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction, followed by detection and quantification using liquid chromatography coupled to mass spectrometry was developed. The analytical method was evaluated for the determination of 23 antibiotics (including parent compounds and some metabolites) in fish, mussels and clams. Recoveries ranged between 30% and 70% for most of the compounds and method detection and quantification limits (MDLs and MQLs) were between 0.01 and 0.31 ng/g dry weigh (dw) and 0.02-1.03 ng/g (dw) respectively. Real seafood samples were analysed using this method. Nine antibiotics were found at levels above MDLs; however none of them exceed the maximum residue limits (MRL) established by the authorities. Tetracycline was the most ubiquitous compound, presenting also the highest concentration: 5.63 ng/g (dw) in fish from Netherlands. In addition, an alternative technique based on microbial growth inhibition was explored as semiquantitative detection method of antibiotics in seafood. This methodology could be applied as a fast screening technique for the detection of macrolides and β-lactams in seafood but further research is needed for other antibiotics families.
供人类食用的海鲜中存在抗生素可能会给消费者带来风险。开发了一种基于QuEChERS（快速、简便、廉价、有效、耐用且安全）萃取法分析海鲜中抗生素的方法，随后使用液相色谱-质谱联用进行检测和定量。该分析方法用于测定鱼类、贻贝和蛤中23种抗生素（包括母体化合物和一些代谢物）。大多数化合物的回收率在30%至70%之间，方法检测限和定量限（MDL和MQL）分别在0.01至0.31 ng/g干重（dw）和0.02 - 1.03 ng/g（dw）之间。使用该方法对实际海鲜样品进行了分析。发现9种抗生素的含量高于检测限；然而，它们均未超过当局规定的最大残留限量（MRL）。四环素是最普遍存在的化合物，其浓度也最高：在荷兰鱼类中为5.63 ng/g（dw）。此外，还探索了一种基于微生物生长抑制的替代技术，作为海鲜中抗生素的半定量检测方法。该方法可作为检测海鲜中大环内酯类和β-内酰胺类抗生素的快速筛选技术，但其他抗生素家族还需要进一步研究。
