Gaur V P, Eldred W, Possin D E, Sarthy P V
Department of Ophthalmology, University of Washington School of Medicine, Seattle.
Cell Tissue Res. 1989 Sep;257(3):497-503. doi: 10.1007/BF00221459.
Monoclonal antibodies that specifically label one or more cell types in retina have been produced; however, only a few antibodies that, in addition, recognize distinct subcellular structures in these cells have been reported. During a search for monoclonal antibodies that bind to specific cell types in the turtle (Pseudemys scripta elegans) retina, we obtained an antibody (20 93; an IgG) that labels the inner segment of cone photoreceptors. Ultrastructural immunocytochemistry using immunogold and avidin/biotin-peroxidase techniques showed that 20 93 antigen is localized to the paraboloid, a region specifically involved in glycogen metabolism in cones. In addition, a few bipolar cells were found to be labeled. The monoclonal antibody showed limited species cross-reactivity and failed to stain mouse, rat, rabbit, dog, cow, Anolis, and human retinas. Immunoblotting showed that monoclonal antibody 20-93 binds to a 40 KDa protein that is present in the retinal membrane. The antibody should be useful in immunological studies of the cone paraboloid.
已经制备出能特异性标记视网膜中一种或多种细胞类型的单克隆抗体；然而，据报道，只有少数抗体能识别这些细胞中不同的亚细胞结构。在寻找能与乌龟（秀丽锦龟）视网膜中特定细胞类型结合的单克隆抗体的过程中，我们获得了一种抗体（20 93；一种IgG），它能标记视锥光感受器的内段。使用免疫金和抗生物素蛋白/生物素-过氧化物酶技术进行的超微结构免疫细胞化学显示，20 93抗原定位于抛物面，这是视锥细胞中专门参与糖原代谢的区域。此外，还发现少数双极细胞被标记。该单克隆抗体显示出有限的种间交叉反应性，不能对小鼠、大鼠、兔子、狗、牛、安乐蜥和人类视网膜进行染色。免疫印迹显示，单克隆抗体20-93与视网膜膜中存在的一种40 kDa蛋白质结合。该抗体在视锥抛物面的免疫学研究中应会有用。
