含降钙素基因相关肽或P物质的明胶微球修复骨质疏松兔的骨缺损。

Gelatin microspheres containing calcitonin gene-related peptide or substance P repair bone defects in osteoporotic rabbits.

作者信息

Chen Jianghao, Liu Wei, Zhao Jinxiu, Sun Cong, Chen Jie, Hu Kaijin, Zhang Linlin, Ding Yuxiang

机构信息

State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center of Oral Diseases, Department of Oral Surgery, School of Stomatology, The Fourth Military Medical University, 145 Western Changle Road, Xi'an, 710032, People's Republic of China.

Department of Stomatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of China.

出版信息

Biotechnol Lett. 2017 Mar;39(3):465-472. doi: 10.1007/s10529-016-2263-4. Epub 2016 Dec 1.

DOI:10.1007/s10529-016-2263-4

PMID:27909823

Abstract

OBJECTIVES

To investigate the therapeutic effect of gelatin microspheres containing different concentrations of calcitonin gene-related peptide (CGRP) or substance P on repairing bone defects in a rabbit osteoporosis model.

RESULTS

Gelatin microspheres containing different concentrations of CGRP or substance P promoted osteogenesis after 3 months in a rabbit osteoporotic bone defective model. From micro-computed tomography imaging results, 10 nM CGRP was optimal for increasing the trabecular number and decreasing the trabecular bone separation degree; similar effects were observed with the microspheres containing 1 µM substance P. Histological analysis showed that the gelatin microspheres containing CGRP or substance P, regardless of the concentration, effectively promoted osteogenesis, and the highest effect was achieved in the groups containing 1 µM CGRP or 1 µM substance P.

CONCLUSIONS

Gelatin microspheres containing CGRP or substance P effectively promoted osteogenesis in a rabbit osteoporotic bone defect model dose-dependently, though their effects in repairing human alveolar ridge defects still need further investigation.

摘要

目的

研究含不同浓度降钙素基因相关肽（CGRP）或P物质的明胶微球对兔骨质疏松模型骨缺损修复的治疗效果。

结果

在兔骨质疏松性骨缺损模型中，含不同浓度CGRP或P物质的明胶微球在3个月后促进了成骨作用。从显微计算机断层扫描成像结果来看，10 nM CGRP在增加小梁数量和降低小梁骨分离程度方面效果最佳；含1 μM P物质的微球也观察到了类似效果。组织学分析表明，含CGRP或P物质的明胶微球，无论浓度如何，均能有效促进成骨作用，且在含1 μM CGRP或1 μM P物质的组中效果最为显著。