Wu Peng, Gao Hui, Liu Jian-Xin, Liu Liang, Zhou Hua, Liu Zhong-Qiu
International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510006, People's Republic of China.
State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macau, People's Republic of China.
Phytochemistry. 2017 Feb;134:122-132. doi: 10.1016/j.phytochem.2016.11.012. Epub 2016 Nov 30.
Seven triterpenoid saponins, named ilexsaponin I-O, along with twelve known ones, were isolated from the roots of Ilex pubescens. The structures of all compounds were elucidated by use of extensive spectroscopic methods (IR, HR-ESI-MS, and 1D and 2D NMR). Sugar residues obtained after acid hydrolysis were identified by TLC and HPLC. The in vitro anti-inflammatory effects of the triterpenoid saponins were also evaluated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Among the isolated saponins, seven compounds were shown to inhibit LPS-induced nitric oxide (NO) and prostaglandin E (PGE) production by suppressing the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), respectively, in LPS-stimulated RAW 264.7 cells. Ilexsaponin I and β-d-glucopyranosyl 3-β-[β-d-xylopyranosyl-(1 → 2)-β-d-glucopyranosyloxy]-olea-12-en-28-oate exerted more potent anti-inflammatory effects than the other compounds tested.
从毛冬青根中分离出7个三萜皂苷，命名为毛冬青皂苷I - O，以及12个已知的三萜皂苷。所有化合物的结构通过广泛的光谱方法（红外光谱、高分辨电喷雾电离质谱以及一维和二维核磁共振）进行了阐明。酸水解后得到的糖残基通过薄层色谱法和高效液相色谱法进行了鉴定。还在脂多糖（LPS）刺激的RAW 264.7巨噬细胞中评估了三萜皂苷的体外抗炎作用。在分离得到的皂苷中，7种化合物分别通过抑制LPS刺激的RAW 264.7细胞中诱导型一氧化氮合酶（iNOS）和环氧化酶 - 2（COX - 2）的表达，显示出抑制LPS诱导的一氧化氮（NO）和前列腺素E（PGE）生成的作用。毛冬青皂苷I和β - d - 吡喃葡萄糖基3 - β - [β - d - 吡喃木糖基 - (1→2) - β - d - 吡喃葡萄糖氧基] - 齐墩果 - 12 - 烯 - 28 - 酸酯比其他测试化合物具有更强的抗炎作用。
