川牛膝根中的三萜皂苷及其对一氧化氮生成的抑制作用。

Triterpenoid saponins from the roots of Cyathula officinalis and their inhibitory effects on nitric oxide production.

作者信息

Jiang Yun-Tao, Yan Wen-Jing, Qi Chu-Lu, Hou Ji-Qin, Zhong Yan-Ying, Li Hui-Jun, Wang Hao, Li Ping

机构信息

State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China; Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China.

State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.

出版信息

Chin J Nat Med. 2017 Jun;15(6):463-466. doi: 10.1016/S1875-5364(17)30069-9.

DOI:10.1016/S1875-5364(17)30069-9

PMID:28629537

Abstract

The present study was designed to investigate the chemical constituents of the roots of Cyathula officinalis. Compounds were isolated by silica gel, Sephadex LH-20, ODS column chromatography, and preparative HPLC. Their structures were determined on the basis of 1D and 2D NMR techniques, mass spectrometry, and chemical methods. One new oleanane-type triterpenoid saponin, 28-O-[α-L-rhamnopyranosyl-(1→3)-β-D-glucuronopyranosyl-(1→3)-β-D-glucopyranosyl] hederagenin (1), was isolated from the roots of Cyathula officinalis. The anti-inflammatory activities of the isolates were evaluated for their inhibitory effects against LPS-induced nitric oxide (NO) production in RAW 264.7 macrophages cells. Compounds 2, 4, and 6 exhibited moderate anti-inflammatory activities.

摘要

本研究旨在调查川牛膝根的化学成分。通过硅胶、葡聚糖凝胶LH-20、ODS柱色谱和制备型高效液相色谱分离化合物。基于一维和二维核磁共振技术、质谱和化学方法确定其结构。从川牛膝根中分离出一种新的齐墩果烷型三萜皂苷，28-O-[α-L-鼠李吡喃糖基-(1→3)-β-D-葡萄糖醛酸吡喃糖基-(1→3)-β-D-葡萄糖吡喃糖基]常春藤皂苷元（1）。评估了分离物对RAW 264.7巨噬细胞中脂多糖诱导的一氧化氮（NO）产生的抑制作用，以评价其抗炎活性。化合物2、4和6表现出中等程度的抗炎活性。