Özbek Nurhayat, Alp Hakan, Çelik Gonca, Ak Tuğba, Çağılcı Osman Can, Yaylı Nurettin, Ocak Ümmühan, Ocak Miraç
Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.
Faculty of Pharmaceutical Sciences, Pharmaceutical Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.
J Fluoresc. 2017 Mar;27(2):635-641. doi: 10.1007/s10895-016-1992-z. Epub 2016 Dec 2.
A chalcone-based Schiff base (5), capable of detecting iron (III) in partially aqueous media, has been designed, then synthesized by the condensation of 3-formyl-2-hydroxyquinoline and acetophenone. To determine iron (III) ion, a simple spectrofluorimetric method was developed by using the synthesized Schiff base. The developed method was validated by analyzing the certified reference material (CRM-SA-C Sandy Soil C). During the process of the determination of iron in food samples, satisfactory accuracy was obtained for spinach and rocket. Nitric acid and hydrogen fluoride were used for the digestion of the certified reference material whereas only nitric acid was used for food samples, in a closed microwave system. Measurements were carried out by using the modified standard addition method. The standard addition graph was linear until 5.0 mg/L. in determination of iron (III). Detection and quantification limits were 0.06 and 0.20 mg/L., respectively. The presented method is simple, time-saving, cost-effective and suitable for determination of iron content of soil and foods.
一种能够在部分水性介质中检测铁（III）的基于查尔酮的席夫碱（5）已被设计出来，然后通过3-甲酰基-2-羟基喹啉与苯乙酮缩合合成。为了测定铁（III）离子，利用合成的席夫碱开发了一种简单的荧光光谱法。通过分析有证标准物质（CRM-SA-C沙质土壤C）对所开发的方法进行了验证。在食品样品中铁的测定过程中，菠菜和芝麻菜的测定结果具有令人满意的准确度。在封闭的微波系统中，使用硝酸和氢氟酸对有证标准物质进行消解，而食品样品仅使用硝酸。采用改进的标准加入法进行测量。在铁（III）的测定中，标准加入曲线在5.0 mg/L之前呈线性。检测限和定量限分别为0.06和0.20 mg/L。所提出的方法简单、省时、经济高效，适用于土壤和食品中铁含量的测定。
