Leguèbe M, Notarangelo M G, Twarogowska M, Natalini R, Poignard C
Max-Planck-Institut für Sonnensystemforschung, Justus-von-Liebig-Weg 3, 37077 Göttingen, Germany.
Istituto per le Applicazioni del Calcolo "M. Picone", Consiglio Nazionale delle Ricerche, Via dei Taurini 19, I-00185 Rome, Italy.
Math Biosci. 2017 Mar;285:1-13. doi: 10.1016/j.mbs.2016.11.015. Epub 2016 Nov 30.
We propose a mathematical model for the transport of DNA plasmids from the extracellular matrix up to the cell nucleus. The model couples two phenomena: the electroporation process, describing the cell membrane permeabilization to plasmids and the intracellular transport enhanced by the presence of microtubules. Numerical simulations of cells with arbitrary geometry, in 2D and 3D, and a network of microtubules show numerically the importance of the microtubules and the electroporation on the effectiveness of the DNA transfection, as observed by previous biological data. The paper proposes efficient numerical tools for forthcoming optimized procedures of cell transfection.
我们提出了一个用于描述DNA质粒从细胞外基质转运至细胞核过程的数学模型。该模型耦合了两种现象：电穿孔过程，用于描述细胞膜对质粒的通透性；以及微管存在时增强的细胞内转运。对具有任意几何形状的二维和三维细胞以及微管网络进行的数值模拟，从数值上显示了微管和电穿孔对DNA转染有效性的重要性，这与先前的生物学数据一致。本文还为即将到来的细胞转染优化程序提出了有效的数值工具。
